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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Microbial Community Diversity and Structure Analysis of Casing Soil During Mushroom (Agaricus bisporus) Growth
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Microbial Community Diversity and Structure Analysis of Casing Soil During Mushroom (Agaricus bisporus) Growth

机译：蘑菇生长过程中套管土壤微生物群落多样性及结构分析

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摘要

The culturable bacterial population, phospholipid fatty acid (PLFA) profile and PCR-DGGE of casing soil were investigated at different mushroom (Agaricus bisporus) cropping stages. The change in soil bacterial PLFAs was always accompanied by a change in the soil culturable bacterial population in the first flush. Comparatively higher bacterial PLFAs and culturable bacterial populations were found in casing soil at the primordia formation stage of the first flush. There was a significant increase in the ratio of fungal to bacterial PLFAs during mushroom growth. Multivariate analysis of PLFA data demonstrated that the mushroom cropping stage could considerably affect the microbial community structure of casing soil. The bacterial population increased significantly from casing soil application to the primordia formation stage of the first flush. Casing soil application resulted in an increase in the ratio of gram-negative bacterial PLFAs to gram-positive bacterial PLFAs, which may suggest that some gramnegative bacteria play an important role in mushroom sporophore initiation. PCR-DGGE of different casing soils showed that the amount of bands in the DGGE map was increased and the strength was enhanced as the mushroom growth. It suggested that the genetic diversity and structure of the bacterial community were affected by the mushroom and these were consistent with the results obtained from the PLFA assays. Based on the observation from the DGGE map of the casing soils, on which the mushroom had high output, the propagation of some bacterial communities which were represented by three special bands was initiated and the result probably signified a choice symbiotic interaction between the mushroom and these bacteria.
机译：研究了不同蘑菇栽培阶段套管土的可培养细菌种群，磷脂脂肪酸（PLFA）图谱和PCR-DGGE。土壤细菌PLFA的变化总是伴随着在第一次冲洗中土壤可培养细菌种群的变化。在初潮的原基形成阶段，在肠衣土壤中发现了相对较高的细菌PLFA和可培养的细菌种群。在蘑菇生长过程中，真菌与细菌PLFA的比例显着增加。 PLFA数据的多变量分析表明，蘑菇种植阶段可能会严重影响套管土的微生物群落结构。从套管土壤施用到第一次潮红的原基形成阶段，细菌种群显着增加。施用套管土壤导致革兰氏阴性细菌PLFA与革兰氏阳性细菌PLFA的比例增加，这可能表明某些革兰氏阴性细菌在蘑菇孢子体的形成中起重要作用。不同套管土壤的PCR-DGGE结果表明，随着蘑菇的生长，DGGE图中的条带数量增加，强度增强。这表明蘑菇影响了细菌群落的遗传多样性和结构，这些与从PLFA分析获得的结果一致。基于蘑菇高产肠衣土壤的DGGE图的观察结果，启动了由三个特殊谱带代表的一些细菌群落的繁殖，其结果可能表明蘑菇与这些菌之间存在选择共生的相互作用。菌。

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