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首页> 外文期刊>Indian Journal of Microbiology >Evaluation of in situ functional activity of casing soils during growth cycle of mushroom (Agaricus bisporus (Lange) Imbach) employing community level physiological profiles (CLPPs)
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Evaluation of in situ functional activity of casing soils during growth cycle of mushroom (Agaricus bisporus (Lange) Imbach) employing community level physiological profiles (CLPPs)

机译：利用群落水平生理概况（CLPPs）评价蘑菇（双孢蘑菇（Lange）Imbach）生长周期中的套管土壤原位功能活性

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摘要

Community level physiological profiles (CLPPs) have been rarely applied to mushroom compost ecosystem, probably for the lack of standardized methodology. Recently, however CLPPs have been employed as a tool to investigate the degree of maturity of compost (Mondini and Insam, 2005, Compost Science and Utilization, 13(1): 27-33). The potential of CLPPs to detect compost maturity test is considerably significant in that it provides sensitivity and the simplicity of the assay. The aim of this work was to investigate the maturity of casing that comprised of farm yard manure and spent compost and influence of casing type on the behaviour of bacterial community during the growth cycle of mushroom Agaricus bisporus (Lange) Imbach employing standardized inoculum density and effects of different data interpretation based on the kinetics of colour formation. Casing samples of different age were extracted at a particular dilution and then inoculated in 96 well microtitre plates. Optical density (OD) in well was measured at 590 nm every 24 hours for 5 days. Principal component analysis (PCA) was performed by employing OD values at fixed average well colour development (AWCD). PCA of fresh samples showed that classification and ordination of samples according to their age were significant with fixed AWCD.
机译：社区水平的生理概况（CLPP）很少应用于蘑菇堆肥生态系统，这可能是由于缺乏标准化的方法。然而，近来，CLPP已被用作研究堆肥成熟度的工具（Mondini和Insam，2005，堆肥科学与利用，13（1）：27-33）。 CLPP检测堆肥成熟度测试的潜力非常重要，因为它提供了测定的灵敏度和简便性。这项工作的目的是利用标准化接种密度和效果，研究蘑菇双孢蘑菇（Lange）Imbach生长周期中由农田肥料和废肥料组成的肠衣的成熟度以及肠衣类型对细菌群落行为的影响。基于颜色形成动力学的不同数据解释。以特定的稀释度提取不同年龄的套管样品，然后接种到96孔微量滴定板中。每24小时在590nm下测量孔中的光密度（OD），持续5天。主成分分析（PCA）通过在固定的平均孔颜色显影（AWCD）下采用OD值进行。新鲜样品的PCA显示，使用固定AWCD时，根据样品的年龄进行分类和排序很重要。

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