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首页> 外文会议>Proceedings of Life in space for life on earth >OPTIMIZING HOST-PATHOGEN IN-FLIGHT ASSAYS FOR C. ELEGANS AND METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
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OPTIMIZING HOST-PATHOGEN IN-FLIGHT ASSAYS FOR C. ELEGANS AND METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

机译：优化对线虫和耐甲氧西林金黄色葡萄球菌的寄主病原体飞行检测

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摘要

This study addresses controls for an assay of bacterialrnvirulence that has been optimized for space flightrnstudies. Caenorhabditis elegans (C. elegans) wormsrningest microorganisms, but are also killed by virulentrnbacteria. Virulence is assessed by the number of bacteriarnsurviving in co-culture with C. elegans, as measuredrnby optical density at 620 nm. Co-cultures ofrnMethicillin-resistant Staphylococcus aureus (MRSA)rnwith C. elegans have a higher OD620 than MRSArngrown alone, which could reflect debris from deadrnworms and/or enhanced growth of the MRSA in responsernto worm-derived factors. The use of mediarnconditioned by pre-incubation with worms demonstratedrnthe presence of temperature-stable factors thatrnchange MRSA growth in a strain-dependent manner.rnSome sources of deionized water contain an undefinedrnantibacterial activity present in conditioned, butrnnot fresh untreated media.
机译：这项研究针对的是针对太空飞行研究进行了优化的细菌毒性测定的对照。秀丽隐杆线虫（C. elegans）蠕虫感染最严重的微生物，但也被病毒性细菌杀死。通过与秀丽隐杆线虫共培养中存活的细菌数来评估毒力，如通过620nm处的光密度测量的。耐甲氧西林金黄色葡萄球菌（MRSA）与秀丽隐杆线虫的共培养比单独生长的MRSA具有更高的OD620，这可能反映死蠕虫的碎屑和/或响应于蠕虫衍生因子的MRSA增长。通过与蠕虫预温育而使用的培养基证明了存在温度稳定因子，这些菌株以应变依赖的方式改变了MRSA的生长。一些去离子水源在未处理但未处理的条件培养基中存在不确定的抗菌活性。

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    Timothy G. Hammond; Holly H. Birdsall; Jeffrey S. Hammond; Patricia L. Allen;

  • 作者单位

    Durham VA Med. Ctr., Research Svc., Durham NC,USA 27705 Nephrology Div., Dept. Int. Med., Duke Univ. Schl. Med., Durham NC,USA 27705 VA Office Research Devel., Washington DC. USA 20420 Timothy.Hammond@va.gov;

    VA Office Research Devel., Washington DC. USA 20420, Depts. Otolaryngology, Immunology and Psychiatry, Baylor Coll. Med., Houston TX,USA 77030 Holly.Birdsall@va.gov;

    The Inst. for Medical Research, Durham NC, USA 27705 jhammond99@gmail.com;

    Durham VA Med. Ctr., Research Svc.,Durham NC,USA 27705 Patricia.Allen@va.gov;

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