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首页> 外文会议>Saratov Fall Meeting 2016: Optical technologies in biophysics amp; medicine XVIII >The counting of native blood cells by digital microscopy
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The counting of native blood cells by digital microscopy

机译：通过数字显微镜计数天然血细胞

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摘要

An algorithm for photographic images processing of blood samples in its native state was developed to determine the concentration of erythrocytes, leukocytes and platelets without individual separate preparation of cells' samples. Special "photo templates" were suggested to use in order to identify red blood cells. The effect of "highlighting" of leukocytes, which was found by authors, was used to increase the accuracy of this type of cells counting. Finally to raise the resolution of platelets from leukocytes the areas of their photo images were used, but not their sizes. It is shown that the accuracy of cells counting for native blood samples may be comparable with the accuracy of similar studies for smears. At the same time the proposed native blood analysis simplifies greatly the procedure of sample preparation in comparison to smear, permits to move from the detection of blood cells ratio to the determination of their concentrations in the sample.
机译：开发了一种用于对原始状态的血液样本进行照片图像处理的算法，无需单独准备细胞样本即可确定红细胞，白细胞和血小板的浓度。建议使用特殊的“照片模板”来识别红细胞。作者发现白细胞“突出显示”的作用被用来增加这种细胞计数的准确性。最后，为了提高白细胞的血小板分辨率，使用了其照片图像的区域，但未使用其大小。结果表明，对天然血样进行计数的细胞的准确性可能与对涂片进行类似研究的准确性相当。同时，与涂片相比，拟议的天然血液分析大大简化了样品制备过程，允许从血细胞比率的检测转向确定样品中血细胞浓度的过程。

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