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The counting of native blood cells by digital microscopy

机译:数字显微镜计算原生血细胞

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An algorithm for photographic images processing of blood samples in its native state was developed to determine the concentration of erythrocytes, leukocytes and platelets without individual separate preparation of cells' samples. Special "photo templates" were suggested to use in order to identify red blood cells. The effect of "highlighting" of leukocytes, which was found by authors, was used to increase the accuracy of this type of cells counting. Finally to raise the resolution of platelets from leukocytes the areas of their photo images were used, but not their sizes. It is shown that the accuracy of cells counting for native blood samples may be comparable with the accuracy of similar studies for smears. At the same time the proposed native blood analysis simplifies greatly the procedure of sample preparation in comparison to smear, permits to move from the detection of blood cells ratio to the determination of their concentrations in the sample.
机译:开发了一种用于摄影图像处理其天然状态的血液样本的算法,以确定红细胞,白细胞和血小板的浓度,而无需单独的细胞样品的单独制备。建议使用特殊的“照片模板”以识别红细胞。作者发现的白细胞的“突出显示”的效果用于提高这种类型的细胞计数的准确性。最后为了从白细胞上提高血小板的分辨率,使用了他们的照片图像的区域,但不是它们的尺寸。结果表明,对本地血液样本计数的细胞的准确性可能与涂片类似研究的准确性相媲美。同时,所提出的本地血液分析大大简化了样品制备的程序与涂抹的允许,允许从血细胞比率的检测到样品中的浓度的测定。

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