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首页> 外文会议>Sensing for agriculture and food quality and safety III >Rapid detection of Salmonella typhimurium on fresh spinach leaves using phage-immobilized magnetoelastic biosensors
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Rapid detection of Salmonella typhimurium on fresh spinach leaves using phage-immobilized magnetoelastic biosensors

机译：使用噬菌体固定的磁弹性生物传感器快速检测新鲜菠菜叶片上的鼠伤寒沙门氏菌

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摘要

This paper presents an investigation into the use of magnetoelastic biosensors for the rapid detection of Salmonella typhimurium on fresh spinach leaves. The biosensors used in this investigation were comprised of a strip-shaped, goldcoated sensor platform (2 mm-long) diced from a ferromagnetic, amorphous alloy and a filamentous fd-tet phage which specifically binds with S. typhimurium. After surface blocking with bovine serum albumin, these biosensors were, without any preceding sample preparation, directly placed on wet spinach leaves inoculated with various concentrations of S. typhimurium. Upon contact with cells, the phage binds S. typhimurium to the sensor thereby increasing the total mass of the sensor. This change in mass causes a corresponding decrease in the sensor's resonant frequency. After 25 min, the sensors were collected from the leaf surface and measurements of the resonant frequency were performed immediately. The total assay time was less than 30 min. The frequency changes for measurement sensors (i.e., phageimmobilized) were found to be statistically different from those for control sensors (sensors without phage), down to 5 × 106 cells/ml. The detection limit may be improved by using smaller, micron-sized sensors that will have a higher probability of contacting Salmonella on the rough surfaces of spinach leaves.
机译：本文介绍了使用磁弹性生物传感器快速检测新鲜菠菜叶上鼠伤寒沙门氏菌的研究。本研究中使用的生物传感器由带状，镀金的传感器平台（2毫米长）组成，该平台从铁磁性非晶合金切成小块，并有丝状fd-tet噬菌体，可特异性结合鼠伤寒沙门氏菌。用牛血清白蛋白表面封闭后，无需任何事先样品制备即可将这些生物传感器直接置于接种了各种浓度鼠伤寒沙门氏菌的湿菠菜叶上。与细胞接触后，噬菌体将鼠伤寒沙门氏菌与传感器结合，从而增加了传感器的总质量。质量的这种变化会导致传感器的共振频率相应降低。 25分钟后，从叶片表面收集传感器，并立即进行共振频率的测量。总测定时间少于30分钟。发现测量传感器（即固定噬菌体）的频率变化与对照传感器（无噬菌体的传感器）的频率变化在统计学上不同，低至5×106细胞/ ml。通过使用较小的，微米大小的传感器可以提高检测极限，该传感器将有较高的可能性将沙门氏菌与菠菜叶片的粗糙表面接触。

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