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首页> 外文会议>Sensing for agriculture and food quality and safety III >The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor
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The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor

机译：基于噬菌体的生物传感器检测蛋壳中的鼠伤寒沙门氏菌

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This paper presents the direct detection of Salmonella typhimurium on shell eggs using a phage-based magnetoelastic (ME) biosensor. The ME biosensor consists of a ME resonator as the sensor platform and E2 phage as the biorecognition element that is genetically engineered to specifically bind with Salmonella typhimurium. The ME biosensor, which is a wireless sensor, vibrates with a characteristic resonant frequency under an externally applied magnetic field. Multiple sensors can easily be remotely monitored. Multiple measurement and control sensors were placed on the shell eggs contaminated by Salmonella typhimurium solutions with different known concentrations. The resonant frequency of sensors before and after the exposure to the spiked shell eggs was measured. The frequency shift of the measurement sensors was significantly different than the control sensors indicating Salmonella contamination. Scanning electron microscopy was used to confirm binding of Salmonella to the sensor surface and the resulting frequency shift results
机译：本文介绍了使用基于噬菌体的磁弹性（ME）生物传感器直接检测带壳蛋中的鼠伤寒沙门氏菌。 ME生物传感器由作为传感器平台的ME共振器和作为生物识别元件的E2噬菌体组成，该E2噬菌体经过基因工程改造以与鼠伤寒沙门氏菌特异性结合。 ME生物传感器是一种无线传感器，它在外部施加的磁场下以特定的共振频率振动。多个传感器可以轻松地进行远程监控。将多个测量和控制传感器放置在被不同浓度的鼠伤寒沙门氏菌溶液污染的带壳鸡蛋上。测量了暴露于加壳蛋之前和之后传感器的共振频率。测量传感器的频移与指示沙门氏菌污染的控制传感器明显不同。扫描电子显微镜用于确认沙门氏菌与传感器表面的结合以及由此产生的频移结果

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