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Detecting peanuts inoculated with toxigenic and atoxienic Aspergillus flavus strains with fluorescence hyperspectral imagery

机译:用荧光高光谱图像检测产毒和无毒的黄曲霉菌株接种的花生

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摘要

Aflatoxin contamination in peanut products has been an important and long-standing problem around the world. Produced mainly by Aspergillus flavus and Aspergillus parasiticus, aflatoxins are the most toxic and carcinogenic compounds among toxins. This study investigated the application of fluorescence visible near-infrared (VNIR) hyperspectral images to assess the spectral difference between peanut kernels inoculated with toxigenic and atoxigenic inocula of A. flavus and healthy kernels. Peanut kernels were inoculated with NRRL3357, a toxigenic strain of A. flavus, and AF36, an atoxigenic strain of A. flavus, respectively. Fluorescence hyperspectral images under ultraviolet (UV) excitation were recorded on peanut kernels with and without skin. Contaminated kernels exhibited different fluorescence features compared with healthy kernels. For the kernels without skin, the inoculated kernels had a fluorescence peaks shifted to longer wavelengths with lower intensity than healthy kernels. In addition, the fluorescence intensity of peanuts without skin was higher than that of peanuts with skin (10 times). The fluorescence spectra of kernels with skin are significantly different from that of the control group (p<0.001). Furthermore, the fluorescence intensity of the toxigenic, AF3357 peanuts with skin was lower than that of the atoxigenic AF36 group. Discriminate analysis showed that the inoculation group can be separated from the controls with 100% accuracy. However, the two inoculation groups (AF3357 vis AF36) can be separated with only ~80% accuracy. This study demonstrated the potential of fluorescence hyperspectral imaging techniques for screening of peanut kernels contaminated with A. flavus, which could potentially lead to the production of rapid and non-destructive scanning-based detection technology for the peanut industry.
机译:花生产品中的黄曲霉毒素污染已成为世界范围内一个重要且长期存在的问题。黄曲霉毒素主要由黄曲霉和寄生曲霉产生,是毒素中毒性最大,致癌的化合物。这项研究调查了荧光可见近红外(VNIR)高光谱图像的应用,以评估黄曲霉的产毒和产毒接种物接种的花生仁与健康仁之间的光谱差异。分别用黄曲霉的产毒菌株NRRL3357和黄曲霉的产毒菌株AF36接种花生仁。在有和没有皮肤的花生仁上记录了在紫外线(UV)激发下的荧光高光谱图像。与健康谷物相比,被污染的谷物表现出不同的荧光特征。对于没有皮肤的谷粒,接种的谷粒的荧光峰移到更长的波长,强度比健康的谷粒低。另外,无皮花生的荧光强度比有皮花生的荧光强度高(10倍)。带有皮肤的谷粒的荧光光谱与对照组的光谱有显着差异(p <0.001)。此外,具有皮肤的产毒素AF3357花生的荧光强度低于产毒素AF36组的荧光强度。区别分析表明,接种组可以100%的准确度与对照组分离。但是,两个接种组(AF3357与AF36)的分离精度仅为〜80%。这项研究证明了荧光高光谱成像技术在筛查受黄曲霉污染的花生仁方面的潜力,这有可能导致为花生行业生产基于快速,无损扫描的检测技术。

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  • 来源
  • 会议地点 Anaheim(US)
  • 作者单位

    Mississippi State University/Geosystems Research Institute, Building 1021, Stennis Space Center, MS, USA 39529,Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, 2 Yuanmingyuan West Rd., Beijing, China 100193,USDA-ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA, USA 70124;

    Mississippi State University/Geosystems Research Institute, Building 1021, Stennis Space Center, MS, USA 39529;

    Mississippi State University/Geosystems Research Institute, Building 1021, Stennis Space Center, MS, USA 39529;

    Mississippi State University/Geosystems Research Institute, Building 1021, Stennis Space Center, MS, USA 39529;

    Mississippi State University/Geosystems Research Institute, Building 1021, Stennis Space Center, MS, USA 39529;

    USDA-ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA, USA 70124;

    USDA-ARS, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA, USA 70124;

    Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, 2 Yuanmingyuan West Rd., Beijing, China 100193;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aflatoxin; Peanut kernel; Classification; Fluorescence; Hyperspectral imaging;

    机译:黄曲霉毒素;花生仁;分类;荧光;高光谱成像;

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