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首页> 外文会议>Sensing for Agriculture and Food Quality and Safety IX >Characterization of E coli biofim formations on baby spinach leaf surfaces using hyperspectral fluorescence imaging
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Characterization of E coli biofim formations on baby spinach leaf surfaces using hyperspectral fluorescence imaging

机译：使用高光谱荧光成像技术表征菠菜叶片上大肠杆菌生物膜的形成

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摘要

Bacterial biofilm formed by pathogens on fresh produce surfaces is a food safety concern because the complex extracellular matrix in the biofilm structure reduces the reduction and removal efficacies of washing and sanitizing processes such as chemical or irradiation treatments. Therefore, a rapid and nondestructive method to identify pathogenic biofilm on produce surfaces is needed to ensure safe consumption of fresh, raw produce. This research aimed to evaluate the feasibility of hyperspectral fluorescence imaging for detecting Escherichia.coli (ATCC 25922) biofilms on baby spinach leaf surfaces. Samples of baby spinach leaves were immersed and inoculated with five different levels (from 2.6×10~4 to 2.6×10~8 CFU/mL) of E.coli and stored at 4℃ for 24 h and 48 h to induce biofilm formation. Following the two treatment days, individual leaves were gently washed to remove excess liquid inoculums from the leaf surfaces and imaged with a hyperspectral fluorescence imaging system equipped with UV-A (365 nm) and violet (405 nm) excitation sources to evaluate a spectral-image-based method for biofilm detection. The imaging results with the UV-A excitation showed that leaves even at early stages of biofilm formations could be differentiated from the control leaf surfaces. This preliminary investigation demonstrated the potential of fluorescence imaging techniques for detection of biofilms on leafy green surfaces.
机译：新鲜产品表面上的病原体形成的细菌生物膜是食品安全问题，因为生物膜结构中复杂的细胞外基质降低了清洗和消毒过程（例如化学或辐射处理）的减少和去除效率。因此，需要一种快速且无损的方法来鉴定农产品表面上的致病生物膜，以确保安全食用新鲜的农产品。这项研究旨在评估高光谱荧光成像检测婴儿菠菜叶表面上的大肠杆菌（ATCC 25922）生物膜的可行性。将菠菜幼叶样品浸没并接种五种不同水平（从2.6×10〜4到2.6×10〜8 CFU / mL）的大肠杆菌，并在4℃下保存24小时和48小时以诱导生物膜形成。在两天的处理之后，轻轻清洗各个叶片，以去除叶片表面多余的液体接种物，然后用配备有UV-A（365 nm）和紫罗兰（405 nm）激发源的高光谱荧光成像系统成像，以评估光谱-基于图像的生物膜检测方法。 UV-A激发的成像结果表明，即使在生物膜形成的早期阶段，叶片也可以与对照叶片表面区分开。这项初步研究证明了荧光成像技术在检测绿叶表面生物膜方面的潜力。

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  • 来源
  • 会议地点 Anaheim(US)
  • 作者

    Hyunjeong Cho; Insuck Baek; Mirae Oh; Sungyoun Kim; Hoonsoo Lee; Moon S. Kim;

  • 作者单位

    Experiment and Research Institute, National Agricultural Products Quality Management Service, 141, Younjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Korea,Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.;

    Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.;

    Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.;

    Experiment and Research Institute, National Agricultural Products Quality Management Service, 141, Younjeon-ro, Gimcheon-si, Gyeongsangbuk-do 39660, Korea;

    Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.;

    Environmental Microbial and Food Safety Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bacterial biofilm; foodborne pathogens; produce safety; hyperspectral fluorescence imaging;

    机译：细菌生物膜；食源性病原体；安全生产；高光谱荧光成像;

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