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Application of a LED-based reflectance sensor for assessing in situ the lycopene content of tomatoes (Lycopersicon esculentum Mill.)

机译:基于LED的反射率传感器在番茄中番茄红素含量评估中的应用(Lycopersicon esculentum Mill。)

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摘要

Nondestructive in situ determination of the antioxidant lycopene of fresh tomato fruits is of large interest for the growers, willing to optimize the harvest time for high quality products. For this, we developed a portable LED-based colorimeter which was able to measure reflectance spectra of whole tomatoes in the 400-750 nm range. The tomato skins from the same samples were then frozen in liquid nitrogen, extracted with an acetone/ethanol/hexane mixture and analyzed by means of a spectrophotometer for their lycopene content. Concentration of lycopene was varying between 70 and 550 mg/Kg fresh weight skin. Partial Least Square regression was used to correlate spectral data to the tomato lycopene content. The multivariate processing of the reflectance data showed that lycopene content could be nicely predicted with a coefficient of determination R~2=0.945 and a root mean square error of cross-validation RMSECV=57 mg/Kg skin fresh weight. These results suggest that portable, low-cost and compact LED-based sensors appear to be promising instruments for the nondestructive assessment of tomato lycopene even in the field.
机译:新鲜番茄果实的抗氧化番茄红素的非破坏性原位测定对于种植者来说非常重要,他们愿意优化高质量产品的收获时间。为此,我们开发了基于LED的便携式色度计,该色度计能够测量整个番茄在400-750 nm范围内的反射光谱。然后将来自相同样品的番茄皮冷冻在液氮中,用丙酮/乙醇/己烷混合物萃取,并通过分光光度计分析其番茄红素含量。番茄红素的浓度在70至550 mg / Kg鲜重皮肤之间变化。使用偏最小二乘回归将光谱数据与番茄番茄红素含量相关联。反射率数据的多变量处理表明,番茄红素含量可以很好地预测,测定系数R〜2 = 0.945,交叉验证的均方根误差RMSECV = 57 mg / Kg皮肤鲜重。这些结果表明,即使在现场,便携式,低成本且紧凑的基于LED的传感器似乎也是用于番茄番茄红素无损评估的有前途的工具。

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  • 来源
  • 会议地点 Baltimore MD(US)
  • 作者单位

    CNR-Istituto di Fisica Applicata 'Nello Carrara' Via Madonna del Piano, 10-50019 Sesto Fiorentino (FI), Italy;

    CNR-Istituto di Fisica Applicata 'Nello Carrara' Via Madonna del Piano, 10-50019 Sesto Fiorentino (FI), Italy;

    CNR-Istituto di Fisica Applicata 'Nello Carrara' Via Madonna del Piano, 10-50019 Sesto Fiorentino (FI), Italy;

    CNR-Istituto di Fisica Applicata 'Nello Carrara' Via Madonna del Piano, 10-50019 Sesto Fiorentino (FI), Italy;

    CNR-Istituto di Fisica Applicata 'Nello Carrara' Via Madonna del Piano, 10-50019 Sesto Fiorentino (FI), Italy;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Tomato; lycopene; colorimetry; LED; chemometrics; PLS;

    机译:番茄;番茄红素比色法发光二极管;化学计量学PLS;

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