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首页> 外文会议>Sensing for Agriculture and Food Quality and Safety VIII >Alternative Soaking Media for the FDA Procedure in the Detection of Salmonella from Tomatoes and Spinach Leaf using Phage Magnetoelastic Biosensors
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Alternative Soaking Media for the FDA Procedure in the Detection of Salmonella from Tomatoes and Spinach Leaf using Phage Magnetoelastic Biosensors

机译：FDA使用噬菌体磁弹性生物传感器从番茄和菠菜叶中检出沙门氏菌的其他浸泡介质

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摘要

Efforts were made to incorporate the phage Magnetoelastic (ME) biosensor in FDA's Spinach Soaking procedures according to FDA 2015 BAM method. Three soaking materials (Lactose broth, LB broth, and Peptone water) and various soaking times were investigated. Using merely 100 Salmonella cells spiked on the produce surfaces, the phage biosensors detected Salmonella within 5 hours when soaking tomatoes in LB broth as opposed to taking up to 24 hours. Salmonella was detected on spinach leaves within 7 hours. These phage ME biosensors provide a promising rapid detection platform using LB broth in FDA's soaking procedures while shortening the incubation period.
机译：根据FDA 2015 BAM方法，已努力将噬菌体磁弹性（ME）生物传感器纳入FDA的菠菜浸泡程序中。研究了三种浸泡材料（乳糖肉汤，LB肉汤和蛋白ept水）和各种浸泡时间。噬菌体生物传感器仅使用刺入农产品表面的100株沙门氏菌细胞，将西红柿浸入LB肉汤中后要花24小时才能在5小时内检测到沙门氏菌。 7小时内在菠菜叶上检测到沙门氏菌。这些噬菌体ME生物传感器在FDA的浸泡程序中使用LB肉汤提供了一个有希望的快速检测平台，同时缩短了孵育时间。

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