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首页> 外文会议>Sensing technologies for global health, military medicine, disaster response, and environmental monitoring; and Biometric technology for human identification VIII >Massively multiplexed microbial identification using resequencing DNA microarrays for outbreak investigation
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Massively multiplexed microbial identification using resequencing DNA microarrays for outbreak investigation

机译：使用重测序DNA微阵列对暴发调查进行大规模多重微生物鉴定

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Multiplexed microbial diagnostic assays are a promising method for detection and identification of pathogens causing syndromes characterized by nonspecific symptoms in which traditional differential diagnosis is difficult. Also such assays can play an important role in outbreak investigations and environmental screening for intentional or accidental release of biothreat agents, which requires simultaneous testing for hundreds of potential pathogens. The resequencing pathogen microarray (RPM) is an emerging technological platform, relying on a combination of massively multiplex PCR and high-density DNA microarrays for rapid detection and high-resolution identification of hundreds of infectious agents simultaneously. The RPM diagnostic system was deployed in Sierra Leone, West Africa in collaboration with Njala University and Mercy Hospital Research Laboratory located in Bo. We used the RPM-Flu microarray designed for broad-range detection of human respiratory pathogens, to investigate a suspected outbreak of avian influenza in a number of poultry farms in which significant mortality of chickens was observed. The microarray results were additionally confirmed by influenza specific real-time PCR. The results of the study excluded the possibility that the outbreak was caused by influenza, but implicated Klebsiella pneumoniae as a possible pathogen. The outcome of this feasibility study confirms that application of broad-spectrum detection platforms for outbreak investigation in low-resource locations is possible and allows for rapid discovery of the responsible agents, even in cases when different agents are suspected. This strategy enables quick and cost effective detection of low probability events such as outbreak of a rare disease or intentional release of a biothreat agent.
机译：多重微生物诊断测定法是检测和鉴定引起以非特异性症状为特征的综合症的病原体的有前途的方法，在传统诊断中很难。同样，此类分析在暴发调查和环境筛选中是否有意释放生物威胁剂中也可以发挥重要作用，这需要同时测试数百种潜在病原体。重测序病原体微阵列（RPM）是一个新兴的技术平台，它依赖于大规模多重PCR和高密度DNA微阵列的组合来同时快速检测和高分辨率鉴定数百种传染原。 RPM诊断系统已与Njala大学和位于Bo的Mercy Hospital Research Laboratory合作在西非塞拉利昂部署。我们使用了专为人类呼吸道病原体的广泛检测而设计的RPM-Flu芯片，以调查在许多观察到鸡死亡率很高的家禽场中疑似禽流感的爆发。另外，通过流感特异性实时PCR证实了微阵列结果。研究结果排除了爆发是由流感引起的可能性，但与肺炎克雷伯菌有关。这项可行性研究的结果证实，在资源不足的地方，可以将广谱检测平台用于暴发调查，并且即使在怀疑有不同的病原体的情况下，也可以快速发现负责的病原体。这种策略可以快速，经济高效地检测低概率事件，例如罕见疾病的爆发或生物威胁剂的有意释放。

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