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首页> 外文会议>Smart Medical and Biomedical Sensor Technology II; Progress in Biomedical Optics and Imaging; vol.5, no.32 >FRET-Based Luminescence Sensors for Carbohydrates and Glycoproteins Analysis
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FRET-Based Luminescence Sensors for Carbohydrates and Glycoproteins Analysis

机译：基于FRET的发光传感器用于碳水化合物和糖蛋白分析

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摘要

This paper describes the development of novel particle-based fluorescence resonance energy transfer (FRET) biosensors. It describes the fundamentals of FRET in heterogeneous systems and the application of the new sensors in monitoring the binding affinity of carbohydrates and glycoproteins to lectins, which are carbohydrate binding proteins. The sensing approach is based on FRET between fluorescein (donor) labeled lectin molecules, adsorbed on the surface of micrometric polymeric beads, and polymeric dextran molecules labeled with Texas Red (acceptor). The FRET signal of the sensor decreases in the presence of carbohydrates or glycoproteins that inhibit the binding of Texas Red-labeled dextran molecules to the lectinic binding sites. The new FRET sensors could discriminate between carbohydrates and glycoproteins based on their binding affinity to the FRET sensing particles. Thery were also used for quantitative analysis of carbohydrates and glycoproteins in aqueous samples.
机译：本文介绍了新型的基于粒子的荧光共振能量转移（FRET）生物传感器的发展。它描述了FRET在异构系统中的基础知识，以及新传感器在监测碳水化合物和糖蛋白与凝集素（作为碳水化合物结合蛋白）的结合亲和力中的应用。传感方法基于吸附在微米级聚合珠表面上的荧光素（供体）标记的凝集素分子与德克萨斯州红（受体）标记的聚合葡聚糖分子之间的FRET。在碳水化合物或糖蛋白的存在下，传感器的FRET信号会降低，而碳水化合物或糖蛋白会抑制德克萨斯红标记的葡聚糖分子与血凝素结合位点的结合。新的FRET传感器可以根据碳水化合物和糖蛋白与FRET感应颗粒的结合亲和力来区分它们。 Thery还用于定量分析水性样品中的碳水化合物和糖蛋白。

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