Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

机译：斜入射反射率差异和寡核苷酸和IgG蛋白微阵列的荧光成像。

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

We have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.

机译：我们构建了斜入射反射率差（OI-RD）显微镜，用于在标准玻璃基板上对DNA和蛋白质微阵列进行无荧光标记的成像。使用OI-RD和荧光图像，我们证明沉积在醛衍生化的玻璃表面上的标记和未标记IgG蛋白分子的润湿行为存在差异。荧光标记剂影响蛋白质特性的潜力凸显了对无标记微阵列检测技术的需求，以补充现有的荧光方法。在印刷和洗涤程序后，我们还展示了寡核苷酸微阵列的OI-RD图像，以证明OI-RD用于在处理过程中微阵列光学特性变化的无损监测。

著录项

来源
《Symposium on Biomicroelectromechanical Systems(BioMEMS); 20030422-20030425; San Francisco,CA; US》|2003年|P.161-166|共6页
会议地点 San Francisco CA(US);San Francisco CA(US);San Francisco CA(US)
作者
James P. Landry; X. D. Zhu; X. W. Guo; J. P. Gregg;
展开▼
作者单位

Department of Physics, University of California at Davis One Shields Avenue, Davis, CA 95616;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;
关键词

相似文献

外文文献
中文文献
专利

1. Label-free detection of oligonucleotide microarrays by oblique-incidence reflectivity difference method [J] . Xu Wang, Kun Yuan, Heng Lu, Journal of Applied Physics . 2010,第6期

机译：斜入射反射率差法无标记检测寡核苷酸微阵列
2. Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method [J] . DAI Jun, LI Lin, WANG JingYi, 中国科学:物理学力学天文学（英文版） . 2012,第012期

机译：斜发射反射差法通过蛋白质微阵列的无标记检测重复性
3. Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method [J] . Heng Lu, JuanWen, XuWang, Journal of optics, A. Pure and applied optics: journal of the European Optical Society . 2010,第9期

机译：斜入射反射率差法检测蛋白质微阵列上的特异性结合
4. Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays [C] . James P. Landry, X. D. Zhu, X. W. Guo, Materials Research Society Symposium . 2003

机译：寡核苷酸和IgG蛋白微阵列的斜发射反射差和荧光成像
5. Label-free detection of biomolecular interactions in microarray format using oblique-incidence reflectivity difference microscopes [D] . Sun, Yung-Shin 2010

机译：使用斜发射反射差异显微镜无标记的微阵列形式的生物分子相互作用的检测
6. Oblique-Incidence Reflectivity Difference Microscope for Label-Free High-Throughput Detection of Biochemical Reactions in Microarray Format [O] . X.D. Zhu, J. P. Landry, Y.S. Sun, -1

机译：斜入射反射率差异显微镜用于微阵列形式的生化反应的无标记高通量检测
7. Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method [O] . Yue Sun, LiPing He, Jun Dai, 2012

机译：斜入射反射率差法无标记实时检测IgG蛋白的特异性结合

Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

摘要

著录项

相似文献

相关主题

期刊订阅