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Fingerprinting technique of Chinese Traditional Medicine and Its Applications

机译:中药指纹图谱技术及其应用

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摘要

The optimal conditions for extraction, isolation and determination of chemical components of Gastrodia tuber with HPLC (High-performance liquid chromatography) were established. Using these conditions the chemical fingerprints and Gastrodin content of Gastrodia tuber samples from 6 different provinces or regions were determined. The results show that the chemical fingerprints of all tested Gastrodia tubers have five common peaks with very approximate retention time. The chemical fingerprints of Gastrodia tuber from the same province are quite similar, but those of Gastrodia tubers from the different provinces are not. Several better Gastrodia tuber populations were found with higher gastrodin content. The findings can be used to identify the quality of Gastrodia tuber, authenticate genuine Gastrodia tuber, determine the Gastrodine content and discover the better Gastrodia tuber populations. The same techniques may be applied to similar research of other medicinal plants.
机译:建立了高效液相色谱法提取,分离和测定天麻块茎化学成分的最佳条件。使用这些条件,测定了来自6个不同省或地区的天麻块茎样品的化学指纹图和天麻素含量。结果表明,所有测试的天麻块茎的化学指纹图都有五个共同的峰,保留时间非常接近。同一省的天麻块茎的化学指纹十分相似,但不同省份的天麻块茎的化学指纹相似。发现天麻中含量更高的几个更好的天麻块茎种群。该发现可用于鉴定天麻块茎的质量,鉴定真正的天麻块茎,确定天麻碱含量并发现更好的天麻块茎种群。相同的技术可以应用于其他药用植物的类似研究。

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