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Use of the optical quadrature method in tomographic microscopy

机译:光学正交方法在断层扫描显微镜中的使用

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摘要

Abstract: The optical quadrature imaging technique, as derived and extended from microwave and laser radar quadrature detection techniques, provides an efficient method for obtaining phase information from a sample that has little or no amplitude contrast. We are able to resolve internal structures of a sample that are defined by relatively small refractive index differences without the use of dyes or stains, while using much lower light levels than conventional techniques. We have constructed a prototype system for imaging microscopic phase- only objects. In this paper, we present its capabilities, as well as the imaging and reconstruction methods used to obtain quantitative information about a sample. !2
机译:摘要:从微波和激光雷达正交检测技术衍生和扩展的光学正交成像技术,提供了一种从振幅对比度很小或没有振幅对比度的样本中获取相位信息的有效方法。我们能够解决由相对较小的折射率差异定义的样品的内部结构,而无需使用染料或色斑,同时所使用的光水平比常规技术低得多。我们已经构建了一个原型系统,用于对微观的仅相位物体进行成像。在本文中,我们介绍了其功能以及用于获取有关样品的定量信息的成像和重建方法。 !2

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