• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Time-Resolved Laser Spectroscopy in Biochemistry III >Fluorescence lifetime distributions of diphenylhexatrienylpropionyl-labeled phospholipids in atherogenic lipoproteins
【24h】

Fluorescence lifetime distributions of diphenylhexatrienylpropionyl-labeled phospholipids in atherogenic lipoproteins

机译：动脉粥样硬化脂蛋白中二苯基己三烯丙基丙酰基标记的磷脂的荧光寿命分布

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: Fluorescent analogs of phosphatidylcholine (PC) and sphingomyelin (SM) were prepared and incorporated into the surface layer of human low-density lipoprotein (LDL) and lipoprotein (a) $LB@Lp(a)$RB@. Diphenylhexatrienylpropionic acid (DPH) was covalently linked to PC (DPH-PC) and SM (DPH-SM). Fluorescence lifetimes of the labeled lipoproteins were determined by phase and modulation fluorometry. Bimodal Lorentzian distributions for the decay times of DPH-PC and DPH-SM in LDL and Lp(a) were found. Lifetime distribution centers for labeled lipids were very similar except for DPH-PC in Lp(a) which was shifted to longer lifetimes. Thus, PC experiences a less polar environment in Lp(a) than in LDL. The distributional width of DPH-PC in Lp(a) was broader than in LDL. Accordingly, phosphatidylcholine must be localized in a much more homogeneous environment in LDL as compared with Lp(a). On the other hand, no difference in distributional widths was observed for DPH-SM in both lipoproteins, showing that SM organization in Lp(a) is unaffected by apo(a). From the obtained fluorescence data, it is proposed that apoprotein B preferentially associates with phosphatidylcholine. !27
机译：摘要：制备了磷脂酰胆碱（PC）和鞘磷脂（SM）的荧光类似物，并将其掺入人低密度脂蛋白（LDL）和脂蛋白（a）$ LB @ Lp（a）$ RB @的表面层中。二苯基己三烯基丙酸（DPH）与PC（DPH-PC）和SM（DPH-SM）共价连接。标记的脂蛋白的荧光寿命通过相位和调制荧光法测定。发现在LDL和Lp（a）中DPH-PC和DPH-SM衰减时间的双峰洛伦兹分布。标记脂质的寿命分配中心非常相似，除了Lp（a）中的DPH-PC转移到更长的寿命。因此，与LDL相比，PC在Lp（a）中的极性环境更小。 Lp（a）中DPH-PC的分布宽度比LDL中的宽。因此，与Lp（a）相比，磷脂酰胆碱必须位于LDL中更为均匀的环境中。另一方面，在两种脂蛋白中均未观察到DPH-SM的分布宽度差异，表明Lp（a）中的SM组织不受apo（a）的影响。根据获得的荧光数据，提出载脂蛋白B优先与磷脂酰胆碱结合。 ！27

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号