染色质重塑复合物亚基hSNF5结合蛋白hNOP17的鉴定及功能初步研究

论文说明：缩写词表

声明

第一部分染色质重塑复合物亚基hSNF5结合蛋白hNOP17的鉴定及功能初步研究

前言

材料与方法

技术路线

实验结果

一、hNOP17表达质粒的构建及鉴定

二、hNOP17与hSNF5的体外结合实验(GST-Pulldown)

三、hNOP17与hSNF5的体内结合实验

四、不同hNOP17删切质粒的构建及表达，与hSNF5的体内结合实验

五、不同hSNF5删切质粒的构建及表达，与hNOP17的体内结合实验

六、hNOP17的组织分布

七、hNOP17多克隆兔抗血清的制备

八、hNOP17与hSNF5的共定位。

九、hNOP17影响hSNF5对p21CIP/WAF1启动子的作用。

讨论

小结

参考文献

Part two: MEKK3 interacting protein in multi-cellular signaling pathway

Abstract

Introduction

Experimental Procedures

Results

Endogenous TRAF7,MEK5 and TAK1 interact with MEKK3

Evidents from FRET(Fluorescence Resonance Energy Transfer) detection confirm the interaction of Mekk3 with TAK1

The phosphorylation and kinase activity were crucial for MEKK3 activate NF-κB reporter gene

TAK1 interacted with MEKK3,but phosphorylated TAK1 did not interact with MEKK3

TAK1 inhibited the effect of MEKK3 on NF-κB promoter activity and regulated MEKK3 phosphorylation.

TAB1 restore the effect of MEKK3 on NF-κB promother activity and regulated MEKK3 phosphorylation.

MEKK3 activated NF-κB promoter in TAK1-/-MEFs

TAB1 but not MEKK3 play important role on regulating TAK1 phosphorylation.

TAB1 regulated the phosphorylation TAK1 and the interaction between MEKK3 and TAK1

MEKK3 interacted with IKKβ

Model of the relation between MEKK3,TAK1 and TAB1

Discussion

Summary

REFERENCES

致谢