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首页> 外文学位 >Early events in the periovulatory interval: Steroidogenesis and proliferation in macaque granulosa cells.
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Early events in the periovulatory interval: Steroidogenesis and proliferation in macaque granulosa cells.

机译：排卵周期的早期事件：猕猴颗粒细胞中的类固醇生成和增殖。

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摘要

The periovulatory interval is defined as the period of time between the ovulatory stimulus and ovulation of the ovarian follicle. It is initiated by a mid-menstrual cycle release of luteinizing hormone (LH) from the pituitary and initiates a cascade of events that eventually lead to extrusion of a fertilizable oocyte as well as remodeling of the follicle into the corpus luteum. Previous experiments looking beyond 12hr after the ovulatory stimulus have identified multiple changes to the preovulatory follicle while little is known of the early periovulatory interval. In spite of the paucity of information available about this time period, it was hypothesized that multiple unknown changes occur early in the interval that are critical to normal ovulation and luteinization. Two endpoints were examined in the periovulatory interval; steroidogenic changes as well as mural granulosa cell proliferation. The novel observation of CYP 21 induction was made as well as identification of 11-deoxycorticosterone (DOC) synthesis in response to hCG both in vivo and in vitro. Additionally, mineralocoritoid receptor (MR) is expressed by granulosa cells thus establishing their potential for corticosteroid sensitivity. Antagonism of MR ablates the normal synthesis of progesterone in response to hCG although the mechanism remains unclear.; It was also concluded that even though mural granulosa cells are less likely to proliferate in response to exogenous stimulus in the form of epidermal growth factor (EGF) after hCG, proliferation can be enforced in even luteinizing granulosa cells using insulin. Moreover, mural granulosa cells express EGF family members in response to hCG and express EGF receptor constitutionally. However, more work needs to be done to elucidate the absence of EGF driven proliferation in luteinizing but not non-luteinized granulosa cells.
机译：排卵周期间隔定义为排卵刺激与卵泡排卵之间的时间间隔。它是由月经周期从垂体释放黄体生成激素（LH）引发的，并引发一系列事件，这些事件最终导致可受精卵母细胞挤出以及卵泡重塑成黄体。在排卵刺激后超过12小时的先前实验中，已发现排卵前卵泡有多个变化，而早期排卵周期的间隔知之甚少。尽管缺乏有关该时间段的可用信息，但据推测在该间隔的早期出现了许多未知变化，这些变化对于正常排卵和黄体化至关重要。在排卵周期中检查了两个终点。类固醇生成的变化以及壁颗粒细胞的增殖。进行了CYP 21诱导的新颖观察，并鉴定了体内和体外响应hCG的11-脱氧皮质酮（DOC）合成。此外，盐皮质激素受体（MR）由颗粒细胞表达，因此确立了其对糖皮质激素敏感性的潜力。 MR的拮抗作用消除了响应hCG的孕激素的正常合成，尽管其机制尚不清楚。还得出结论，即使hCG后壁上的颗粒细胞不太可能以表皮生长因子（EGF）的形式响应外源刺激而增殖，甚至在使用胰岛素的黄化颗粒细胞中也可以促进增殖。而且，壁膜颗粒细胞响应hCG表达EGF家族成员，并在结构上表达EGF受体。然而，需要做更多的工作来阐明在黄素化而不是非黄素化的颗粒细胞中不存在由EGF驱动的增殖。

著录项

  • 作者

    Fru, Karenne Ntsang.;

  • 作者单位

    Medical College of Georgia.;

  • 授予单位 Medical College of Georgia.;
  • 学科 Biology Animal Physiology.
  • 学位 Ph.D.
  • 年度 2008
  • 页码 122 p.
  • 总页数 122
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生理学;
  • 关键词

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