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Changes in sympathetic neurotransmission and growth factor expression in the normal aging rat retina.

机译：正常衰老大鼠视网膜中交感神经传递和生长因子表达的变化。

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摘要

It is estimated that nearly 160 million people worldwide suffer from significant visual impairment. Some of these cases require surgery, some are possibly preventable, while others are incurable. Many diseased states of the eye have been dutifully investigated to see changes in inflammatory markers, growth factor expression and various other protein expression associated with the particular ailment. While we now have a working paradigm of some of these illnesses, we do not have a great understanding of what is to be expected in the normal aging process. This thesis examines some of the changes seen in the normal aging retina and choroid in order to aid in the explanation of the difference between normal ocular aging and ocular diseased states.; Retinal and choroidal research was conducted on 8, 22, and 32 month F344 x BN F1 hybrid male rats. Real time PCR was conducted to check for changes in mRNA, while either immunohistochemistry or Western blot densitometry was conducted for changes in protein expression with age. A decrease in sympathetic neurotransmission of both dopamine beta-hydroxylase and norepinephrine was found with age that may parallel other aging organs. In order to compensate for the decreases seen, the sympathetic receptor, beta1-adrenergic receptor was found to significantly increase with age due to denervation supersensitivity.; Many changes are seen in various states of ocular diseases. However, what changes of growth factor expression are seen in normal aging is unknown. Using the same strain of rats and the same techniques, we investigated changes in growth factor expression in normal aging rat retina. We found an increased expression of the VEGF and its receptor KDR which could lead to angiogenesis, but a decrease in Tie-s which would lead to vessel stabilization. At the same time, the retina was expressing less PEDF. PEDF has not only protective effects on photoreceptor cells, but also slows angiogenesis. The data collected suggests that the retina creates an environment well suited for angiogenesis, and that some diseased states may only be an acceleration of normal aging.
机译：据估计，全世界将近1.6亿人患有严重的视觉障碍。其中某些情况需要手术，有些可能是可以预防的，而另一些则是无法治愈的。我们已认真研究了许多眼部疾病状态，以查看与特定疾病相关的炎症标志物，生长因子表达和各种其他蛋白质表达的变化。虽然我们现在对其中一些疾病有一个有效的范例，但我们对正常的衰老过程中预期的情况还没有很好的了解。本论文研究了正常衰老的视网膜和脉络膜中的一些变化，以帮助解释正常的眼衰老与眼病状态之间的差异。在8、22和32个月F344 x BN F1杂种雄性大鼠上进行了视网膜和脉络膜研究。进行实时PCR以检查mRNA的变化，同时进行免疫组织化学或蛋白质印迹光密度法来检测蛋白质表达随年龄的变化。发现多巴胺β-羟化酶和去甲肾上腺素的交感神经传递减少，其年龄可能与其他衰老器官平行。为了补偿所见的减少，由于去神经超敏反应，发现交感神经受体β1-肾上腺素能受体随着年龄的增长而显着增加。在各种眼病状态中都可以看到许多变化。然而，在正常衰老中看到的生长因子表达的变化尚不清楚。使用相同的大鼠品系和相同的技术，我们研究了正常衰老大鼠视网膜中生长因子表达的变化。我们发现VEGF及其受体KDR的表达增加可能导致血管生成，但Tie-s减少则导致血管稳定。同时，视网膜表达的PEDF较少。 PEDF不仅对感光细胞具有保护作用，而且减缓血管生成。收集到的数据表明，视网膜创造了一个非常适合血管生成的环境，某些疾病可能只是正常衰老的加速。

著录项

  • 作者

    Smith, Christopher P.;

  • 作者单位

    Southern Illinois University at Carbondale.$bMolecular Biology Microbiology and Biochemistry.;

  • 授予单位 Southern Illinois University at Carbondale.$bMolecular Biology Microbiology and Biochemistry.;
  • 学科 Biology Animal Physiology.
  • 学位 M.S.
  • 年度 2007
  • 页码 75 p.
  • 总页数 75
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生理学;
  • 关键词

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