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首页> 外文学位 >Evolutionary based classification of fungal lipases as a framework for structure and function prediction of putative lipases.
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Evolutionary based classification of fungal lipases as a framework for structure and function prediction of putative lipases.

机译：基于进化的真菌脂肪酶分类，作为推定脂肪酶结构和功能预测的框架。

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摘要

Lipases are a family of enzymes which catalyze the hydrolysis of lipids and they exhibit esterase type activities such as phospholipase, lysophospholipase, cutinase and amidase. These enzymes have applications in detergent, the production of oil and fat, baked products, organic synthesis, hard surface cleaning, leather, paper, and cocoa butter. Lipases are versatile and have become a major source of industrial lipolytic enzymes. I performed a comparative analysis of protein sequences of well characterized fungal lipolytic enzymes from SWISS-PROT and developed a classification system to assist structural and functional characterization of newly identified putative lipase gene sequences. The distance-based UPGMA method was used for constructing the phylogenetic tree. Eight sequences of known secondary and tertiary structure were used to predict the secondary structure of similar sequences with unknown structure. Using sixteen different tools from SWISS-PROT and NPS , the secondary structures of sequences with unknown 3-dimensional structures were predicted. The evolutionary based clustering of lipase protein sequences resulted in seven major families with the largest family being divided into five subfamilies and two single member branches. This analysis allowed us to: (i) perform a comparative study of well-characterized fungal lipases to develop a comprehensive classification system for fungal lipases, (ii) assess various protein secondary structure prediction tools to select suitable tool(s) for predicting secondary structures of lipases, (iii) predict structural features important for specific function of lipases such as residues forming the catalytic sites, disulfide bonds and salt bridges, and (iv) develop a framework to predict putative function and reaction conditions of newly identified lipase gene sequences.
机译：脂肪酶是催化脂质水解的酶家族，它们表现出酯酶类型的活性，例如磷脂酶，溶血磷脂酶，角质酶和酰胺酶。这些酶可用于洗涤剂，油脂生产，烘焙产品，有机合成，硬表面清洁，皮革，纸张和可可脂中。脂肪酶用途广泛，已成为工业脂解酶的主要来源。我对来自SWISS-PROT的表征良好的真菌脂解酶的蛋白质序列进行了比较分析，并开发了分类系统，以协助新鉴定的假定脂肪酶基因序列的结构和功能表征。基于距离的UPGMA方法用于构建系统发育树。使用八个已知二级和三级结构的序列来预测结构未知的相似序列的二级结构。使用来自SWISS-PROT和NPS的十六种不同工具，可以预测具有未知三维结构的序列的二级结构。脂肪酶蛋白序列的基于进化的聚类导致七个主要家族，其中最大家族被分为五个亚家族和两个单成员分支。该分析使我们能够：（i）对特性良好的真菌脂肪酶进行比较研究，以开发真菌脂肪酶的全面分类系统，（ii）评估各种蛋白质二级结构预测工具，以选择合适的工具来预测二级结构（iii）预测对于脂肪酶的特定功能重要的结构特征，例如形成催化位点，二硫键和盐桥的残基，以及（iv）建立框架以预测新鉴定的脂肪酶基因序列的假定功能和反应条件。

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  • 作者

    Behdad, Bahareh.;

  • 作者单位

    Concordia University (Canada).;

  • 授予单位 Concordia University (Canada).;
  • 学科 Biology Microbiology.
  • 学位 M.Sc.
  • 年度 2006
  • 页码 134 p.
  • 总页数 134
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

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