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首页> 外文学位 >The N resistance protein recognizes its tobacco mosaic virus elicitor through a novel mechanism.
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The N resistance protein recognizes its tobacco mosaic virus elicitor through a novel mechanism.

机译：N抵抗蛋白通过一种新机制识别其烟草花叶病毒激发子。

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摘要

In their natural environments plants are constantly attacked by a variety of pathogens. Plants have therefore evolved diverse defense mechanisms that allow them to evade the assaults. One strategy used by plants relies on germ-line encoded molecules encoded by Resistance (R) genes that specifically recognize the presence of gene products of corresponding Avirulence (Avr) gene carried by a given pathogen and then initiate defense responses. To date, more than forty R genes, which confer resistance to all classes of plant pathogens, have been cloned. R gene products show striking similarity. They contain a few conserved domains including the TIR, CC, NB and LRR domains. These domains are also found in animal innate immune molecules, especially the TLRs and NODs. The mechanism that R proteins use to recognize pathogen-derived Avr proteins and then to initiate defense signaling was investigated. The tobacco model system, which consists of the tobacco N gene and the tobacco mosaic virus replicase proteins, was used in these studies. N is a TIR-NB-LRR type R protein. Yeast screens with the LRR domain of N and the full length N protein identified several potential interaction partners for N including HSP90, PP5 and SPL transcription. These and proteins that they are known to associate with were examined for their roles in N-mediated resistance using VIGS. All except PP5 were required for N function. N was found to be a cytoplasmic and, to a lesser extent, nuclear protein. The localization of N does not change on infection with TMV or in the presence of its Avr protein. N was also found to associate in vivo with its Avr elicitor, the 50-kDa helicase domain of the viral replicase, p50. This is the first report of an in vivo association between an R and Avr protein. Surprisingly, the TIR domain of N is necessary and sufficient for this association. These data have been integrated into existing models for R-Avr interaction and used to develop a new model for the function of N. The results presented here also suggest novel functions for the conserved TIR domain and indeed for R proteins.
机译：在自然环境中，植物经常受到各种病原体的攻击。因此，植物进化出了多种防御机制，可以逃避攻击。植物使用的一种策略依赖于由抗性（R）基因编码的种系编码分子，该分子特异性识别给定病原体携带的相应无毒（Avr）基因的基因产物的存在，然后启动防御反应。迄今为止，已经克隆了超过四十个赋予所有植物病原体抗性的R基因。 R基因产物显示出惊人的相似性。它们包含一些保守的域，包括TIR，CC，NB和LRR域。这些结构域也存在于动物先天免疫分子中，尤其是TLR和NOD。研究了R蛋白用于识别病原体衍生的Avr蛋白然后启动防御信号转导的机制。在这些研究中使用了由烟草N基因和烟草花叶病毒复制酶蛋白组成的烟草模型系统。 N是TIR-NB-LRR R型蛋白。用N的LRR结构域和全长N蛋白质进行的酵母筛选确定了N的几种潜在相互作用伴侣，包括HSP90，PP5和SPL转录。使用VIGS检查了这些蛋白和与它们相关的蛋白在N介导的抗性中的作用。 N功能需要PP5以外的所有元素。 N被发现是一种细胞质，在较小程度上是核蛋白。 N的定位在感染TMV或存在其Avr蛋白时不会改变。还发现N在体内与其病毒复制酶p50的50kDa解旋酶结构域的Avr激发子缔合。这是R和Avr蛋白之间体内结合的首次报道。出乎意料的是，N的TIR结构域对于这种结合是必要和充分的。这些数据已集成到R-Avr相互作用的现有模型中，并用于开发N功能的新模型。此处显示的结果还暗示了保守TIR结构域以及R蛋白的新功能。

著录项

  • 作者

    Burch-Smith, Tessa Maureen.;

  • 作者单位

    Yale University.;

  • 授予单位 Yale University.;
  • 学科 Biology Molecular.; Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 137 p.
  • 总页数 137
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;植物病理学;
  • 关键词

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