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Gene CATCHR: Gene C&barbelow;loning a&barbelow;nd T&barbelow;agging for C&barbelow;aenorhabditis elegans using yeast H&barbelow;omologous R&barbelow;ecombination: A novel approach for the analysis of gene expression.

机译：基因CATCHR：基因C，利用酵母H和同源线R，形成线粒体：一种新的分析基因表达的方法。

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Expression patterns of gene products provide important insights into gene function. Reporter constructs are frequently used to study gene expression in Caenorhabditis elegans, but the sequence context of a given gene is inevitably altered in such constructs, which may therefore lack important regulatory elements. I describe the development of Gene C ATCHR, a novel method of generating reporter-fusions of worm genes that exploits yeast homologous recombination. Yeast homologous recombination facilitates the cloning of large genomic regions, allowing the isolation of complete open reading frames and regulatory sequences in promoters, untranslated regions and flanking DNA. Gene CATCHR thus preserves the original regulatory context of a given gene as much as possible. Comparisons to genes with known expression patterns validate the use of the Gene CATCHR system. Further, novel expression data was generated. Mutant rescue experiments demonstrate that Gene CATCHR-generated transgenes are functional. Gene CATCHR represents a valuable tool to study gene expression.

机译：基因产物的表达模式提供了对基因功能的重要见解。记者构建体经常用于研究秀丽隐杆线虫中的基因表达，但是给定基因的序列背景在此类构建体中不可避免地发生了变化，因此可能缺乏重要的调控元件。我描述了Gene C ATCHR的发展，这是一种利用酵母同源重组产生蠕虫基因的报告基因融合蛋白的新方法。酵母同源重组促进了大基因组区域的克隆，从而允许在启动子，非翻译区和侧翼DNA中分离出完整的开放阅读框和调控序列。因此，基因CATCHR尽可能保留给定基因的原始调控环境。与具有已知表达模式的基因的比较证实了基因CATCHR系统的使用。此外，产生了新颖的表达数据。突变拯救实验表明，基因CATCHR产生的转基因是有功能的。基因CATCHR代表了研究基因表达的有价值的工具。

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作者
Sassi, Holly Elizabeth.;
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University of Toronto (Canada).;

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授予单位 University of Toronto (Canada).;
学科 Molecular biology.;Genetics.
学位 M.Sc.
年度 2006
页码 143 p.
总页数 143
原文格式 PDF
正文语种 eng
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专利

1. Gene Catchr - Gene cloning and tagging for Caenorhabditis elegans using yeast homologous recombination: a novel approach for the analysis of gene expression [J] . Sassi HE, Renihan S, Spence AM, Nucleic Acids Research . 2005,第18期

机译：Gene Catchr-使用酵母同源重组技术对秀丽隐杆线虫进行基因克隆和标记：分析基因表达的新方法
2. Sentinel interaction mapping – a generic approach for the functional analysis of human disease gene variants using yeast [J] . Barry P. Young, Kathryn L. Post, Jesse T. Chao, Disease models & mechanisms: DMM . 2020,第7期

机译：Sentinel相互作用测绘 - 一种使用酵母功能分析的通用方法
3. PCR fusion-based approach to create reporter gene constructs for expression analysis in transgenic C-elegans [J] . Hobert O BioTechniques . 2002,第4期

机译：基于PCR融合的方法创建报告基因构建体以在转基因C-线虫中进行表达分析
4. Gene Catchr—Gene Cloning And Tagging for Caenorhabditis elegans using yeast Homologous Recombination: a novel approach for the analysis of gene expression [O] . Holly E. Sassi, Stephanie Renihan, Andrew M. Spence, 2005

机译：基因捕获器—使用酵母同源重组技术对秀丽隐杆线虫进行基因克隆和标记：一种用于基因表达分析的新方法
5. Gene Catchr—Gene Cloning And Tagging for Caenorhabditis elegans using yeast Homologous Recombination: a novel approach for the analysis of gene expression [O] . Sassi Holly E., Renihan Stephanie, Spence Andrew M., 100

机译：基因捕获器—使用酵母同源重组技术对秀丽隐杆线虫进行基因克隆和标记：一种用于基因表达分析的新方法

Gene CATCHR: Gene C&barbelow;loning a&barbelow;nd T&barbelow;agging for C&barbelow;aenorhabditis elegans using yeast H&barbelow;omologous R&barbelow;ecombination: A novel approach for the analysis of gene expression.

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