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Evaluation of the Genetic Differences Between Two Subtypes of Campylobacter Fetus (Fetus and Venerealis) in Canada.

机译：在加拿大评估弯曲杆菌胎儿的两种亚型（胎儿和性病）之间的遗传差异。

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摘要

he pathogen Campylobacter fetus (CF) is classified into two subspecies, Campylobacter fetus subspecies fetus (CFF) and Campylobacter fetus subspecies venerealis (CFV). Even though CFF and CFV are genetically closely related, they exhibit differences in their host adaptation; CFF inhabits the gastrointestinal tract of both humans and several animal species, while classical CFV is specific to the bovine genital tract and is of particular concern with respect to international bovine trade regulation. Traditionally, differentiation between the two subspecies has been achieved using a limited number of biochemical tests but more rapid and definitive genetic methods of discrimination are desired. A recent study suggested that the presence of a genomic island only in CFV could discriminate between the two subspecies but this hypothesis could not be confirmed on a collection of isolates originating in Canada.;To identify alternative gene targets that would support accurate subspecies discrimination, this study has applied several approaches including suppression subtractive hybridization and whole genome sequencing supplemented with optical mapping. A subtractive hybridization screen, using a well-characterized CFV isolate recovered during routine screening of bulls in an Artificial Insemination center in western Canada and that lacked much of the genomic island and a typical Canadian CFF isolate, yielded 50 clones; characterization of these clones by hybridization screening against selected CF isolates and by nucleotide sequence BLAST analysis identified three potentially CFV-specific clones that contained inserts originating from a second genomic island. Further screening using a larger CF sample set found that only Clone
机译：病原体胎儿弯曲杆菌（CF）分为两个亚种，即弯曲杆菌胎儿亚种（CFF）和弯曲杆菌胎儿亚种（CFV）。尽管CFF和CFV在遗传上密切相关，但它们在宿主适应性上仍表现出差异。 CFF居住在人类和几种动物的胃肠道中，而经典的CFV特定于牛的生殖道，并且在国际牛贸易法规方面尤其令人关注。传统上，使用有限数量的生化测试已实现了两个亚种之间的区分，但需要更快速和确定的遗传学区分方法。最近的一项研究表明，仅CFV中存在基因组岛可以区分这两个亚种，但这一假说不能在源自加拿大的一系列分离株中得到证实。这项研究应用了几种方法，包括抑制消减杂交和全基因组测序以及光学作图。利用加拿大西部人工授精中心对公牛进行例行筛查时回收的，特性良好的CFV分离株进行的减性杂交筛选，该株缺少大量的基因组岛和典型的加拿大CFF分离株，产生了50个克隆。通过针对所选CF分离物的杂交筛选和核苷酸序列对这些克隆的鉴定，BLAST分析鉴定了三个潜在的CFV特异性克隆，其中包含源自第二个基因组岛的插入片段。使用更大的CF样本集进一步筛选发现只有克隆

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  • 作者

    Mukhtar, Lenah.;

  • 作者单位

    University of Ottawa (Canada).;

  • 授予单位 University of Ottawa (Canada).;
  • 学科 Biology Microbiology.;Health Sciences Immunology.
  • 学位 M.Sc.
  • 年度 2013
  • 页码 161 p.
  • 总页数 161
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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