• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Metabolic engineering of acetate-production pathways for the production of isoamyl acetate and succinate in Escherichia coli.
【24h】

Metabolic engineering of acetate-production pathways for the production of isoamyl acetate and succinate in Escherichia coli.

机译：在大肠杆菌中生产乙酸异戊酯和琥珀酸酯的乙酸生产途径的代谢工程。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Products derived from the central aerobic metabolic pathway in Escherichia coli often compete with the acetate-producing pathways poxB and ackA-pta for glucose. The production of isoamyl acetate from acetyl-CoA by ATF2, a yeast alcohol acetyltransferase, was a model system to demonstrate the beneficial effects of reducing aerobic acetate production. Ester production was 80% higher in the poxB, ackA-pta strain (0.18 mM) than found in the single ackA-pta mutant, when both overexpressed the PDH complex, panK, and ATF2. Anaerobically, succinate was produced alongside isoamyl acetate to maintain the proper redox balance between NADH and NAD+. The two compounds could be easily separated in an industrial setting because of the difference in their volatilities. An ldhA, adhE double mutant strain served as the control strain to test the effect of an additional ackA-pta mutation. The triple mutant produced 36% more ester (9.4 mM) and 7 times more succinate (45.5 mM) than the control.
机译：源自大肠杆菌中性需氧代谢途径的产物通常与乙酸盐产生途径poxB和ackA-pta竞争葡萄糖。由ATF2（一种酵母醇乙酰基转移酶）从乙酰辅酶A生产乙酸异戊酯的模型系统证明了减少乙酸需氧量的有益作用。当过表达PDH复合物，panK和ATF2时，poxB，ackA-pta菌株（0.18 mM）的酯生成量比单个ackA-pta突变体发现的高80％。厌氧地，琥珀酸与乙酸异戊酯一起产生，以维持NADH和NAD +之间的适当氧化还原平衡。由于它们的挥发性不同，两种化合物在工业环境中很容易分离。将ldhA，adhE双突变株用作对照株，以测试另外的ackA-pta突变的作用。该三重突变体产生的酯（9.4 mM）多36％，琥珀酸（45.5 mM）多7倍。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号