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The Association of Interleukin-27 and HIV Infection in Chinese.

机译：白细胞介素27与中国艾滋病毒感染的关系。

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摘要

Human Immunodeficiency Virus (HIV) is the causative agent of Acquired Immunodeficiency Syndrome (AIDS); HIV/AIDS caused 1.8 million deaths world-widely in 2010 and became a major global health challenge. HIV co-infections with Hepatitis B virus (HBV), Hepatitis C virus (HCV) are common and have emerged into new health problems with severe clinical consequences. Since the discovery of HIV, massive progress in understanding of the pathogen has been achieved. Due to the restriction of research model, how human immune system responds to HIV infection, particularly, to HBV or HCV co-infections is still worthy further elucidation.;A cohort study was first conducted in Shenzhen regarding the seroprevalence of HBV, HCV infections among HIV-infected population. Totally 914 HIV positive individuals were recruited in the study and tested for HBsAg and anti-HCV antibodies. The results showed a 10.9% (100/914) HIV/HBV co-infection rate, 14.6% (133/914) HIV/HCV co-infection prevalence and 3.7% (34/914) HIV/HBV/HCV triple-infection prevalence. Multivariate logistic regression revealed that HIV transmission risk behavior was significantly associated with HIV, HBV, HCV co-infections. Most HIV/HBV co-infection cases got HIV through sexual contact including heterosexual and homosexual behaviors (95/100, 95%); while most HIV/HCV co-infection subjects were injection drug users (IDUs) (89/133, 66.9%). In the case of HIV/HBV/HCV triple-infection, IDUs accounted for a large ratio (28/34, 82.4%). Among IDUs, most of them were male (108/122, 88.5%) and nearly half were aged 27 to 32 years old (56/122, 45.9%). Near half people who got HIV through blood and blood products were HIV/HCV co-infected (10/23, 43.5%). Gender has a significant correlation with HIV risk behavior and most IDUs were male.;Next, we applied ELISA to test HIV positive clinical samples and proved that plasma interleukin-27 (IL-27) level was significantly elevated in HIV mono-infected, HIV/HBV co-infected and HIV/HCV co-infected subjects when compared with healthy controls. Later, we further revealed that plasma IL-27 titer was not significantly varied among HIV, HBV and HCV co-infections except between HIV/HCV co-infections and HIV/HBV/HCV triple-infections. We also observed a significant positive correlation between CD4+ T cell counts and plasma IL-27 titer within HIV mono-infected group (r = 0.177, P = 0.034).;We further analyzed the impact HIV and HCV viral loads on plasma IL-27 titer. We found there was no significant correlation between HIV viral load and IL-27 titer among HIV mono-infected individuals (r = - 0.063, P = 0.679); while a significant positive correlation was observed between HIV viral load and IL-27 titer in HIV/HCV co-infected individuals (r = 0.362, P = 0.049). In the case of HIV/HCV co-infection, there was no significant linear correlation between HIV and HCV viral loads (r = - 0.072, P = 0.704) but exist obvious subdivision of samples in terms of HIV and HCV viral loads with significant IL-27 titer variance (P = 0.014). No correlation was observed between HCV viral load and IL-27 titer (r = - 0.119, P = 0.530).;IL-27 p28 polymorphisms were genotyped with TaqManRTM Allelic Discrimination Assay in Chinese men who have sex with men (MSM) population in Shenzhen and the results revealed that proportions of IL-27 p28 -964A/G and 4603G/A genotypes were not significantly different from the healthy controls; IL-27 p28 -964A/G and 4603G/A allele frequencies were similar between HIV positive MSM group and healthy control MSM group. Results also showed that for IL-27 p28 2905T/G polymorphism, TG genotype has a 2.77-fold decreased risk of HIV susceptibility and subjects with G allele has a 2.72-fold decreased risk of HIV susceptibility. Linkage disequilibrium (LD) coefficients were observed between IL-27 p28 -964A/G and 2905T/G (|D'| = 0.942), -964A/G and 4603G/A (|D'| = 0.930). Haplotype analyses revealed that haplotype GGG has a protective role against HIV infection in MSM population (OR = 0.313; P < 0.001).;In conclusion, the seroprevalences of HBV and HCV infection among HIV positive population in Shenzhen were surveyed and risk factors associated with co-infections were analyzed. Plasma IL-27 titer was significantly elevated in HIV mono-infected, HIV/HBV co-infected and HIV/HCV co-infected individuals. IL-27 level was correlated with CD4+ T cell counts within HIV mono-infected people. A significant positive correlation was found between HIV viral load and IL-27 titer in HIV/HCV co-infected individuals (r = 0.362, P = 0.049). IL-27 p28 2905T/G was associated with individual susceptibility to HIV infection and haplotype GGG showed a protective role in restricting HIV infection in MSM population.
机译：人类免疫缺陷病毒（HIV）是获得性免疫缺陷综合症（AIDS）的病原体； 2010年，艾滋病毒/艾滋病在全球造成180万人死亡，并成为全球主要的健康挑战。 HIV与乙型肝炎病毒（HBV），丙型肝炎病毒（HCV）的共同感染很常见，并已出现新的健康问题，并带来严重的临床后果。自从发现艾滋病毒以来，在了解病原体方面已取得了巨大进展。由于研究模型的局限性，人类免疫系统如何应对HIV感染，特别是对HBV或HCV合并感染仍有待进一步阐明。;首次在深圳进行了一项关于HBV血清阳性率，HCV感染人群的队列研究受HIV感染的人群。该研究共招募了914名HIV阳性患者，并测试了HBsAg和抗HCV抗体。结果显示HIV / HBV合并感染率为10.9％（100/914），HIV / HCV合并感染率为14.6％（133/914）和HIV / HBV / HCV三重感染率为3.7％（34/914） 。多元逻辑回归分析表明，HIV传播风险行为与HIV，HBV，HCV合并感染显着相关。大多数HIV / HBV合并感染病例都是通过性接触而感染HIV，包括异性恋和同性恋行为（95/100，95％）；而大多数HIV / HCV合并感染的受试者是注射吸毒者（89/133，66.9％）。在HIV / HBV / HCV三重感染的情况下，注射毒品者占很大的比例（28 / 34，82.4％）。在注射毒品使用者中，大多数是男性（108 / 122，88.5％），近一半年龄在27至32岁之间（56 / 122，45.9％）。通过血液和血液制品感染艾滋病毒的人中，有将近一半是HIV / HCV共同感染者（10 / 23，43.5％）。性别与HIV危险行为有显着相关，大多数IDU是男性。接下来，我们使用ELISA对HIV阳性临床样本进行了测试，并证明了单感染HIV的血浆IL-27（IL-27）水平明显升高与健康对照相比，/ HBV合并感染的受试者和HIV / HCV合并感染的受试者。后来，我们进一步揭示，除了HIV / HCV合并感染和HIV / HBV / HCV三重感染之间，血浆IL-27滴度在HIV，HBV和HCV合并感染之间没有显着差异。我们还观察到HIV单一感染组中CD4 + T细胞计数与血浆IL-27滴度之间存在显着正相关（r = 0.177，P = 0.034）。；我们进一步分析了HIV和HCV病毒载量对血浆IL-27的影响。效价。我们发现，在HIV单一感染者中，HIV病毒载量与IL-27滴度之间无显着相关性（r =-0.063，P = 0.679）。而在HIV / HCV合并感染者中，HIV病毒载量与IL-27滴度之间存在显着正相关（r = 0.362，P = 0.049）。在HIV / HCV合并感染的情况下，HIV和HCV病毒载量之间无显着线性相关性（r =-0.072，P = 0.704），但存在明显IL的HIV和HCV病毒载量样本明显细分-27滴度差异（P = 0.014）。没有观察到HCV病毒载量与IL-27滴度之间的相关性（r =-0.119，P = 0.530）;使用TaqManRTM等位基因鉴别分析对IL-27 p28基因多态性进行了基因分型。深圳的研究结果显示，IL-27 p28 -964A / G和4603G / A基因型的比例与健康对照组无显着性差异。 HIV阳性MSM组和健康对照组MSM组之间的IL-27 p28 -964A / G和4603G / A等位基因频率相似。结果还显示，对于IL-27 p28 2905T / G多态性，TG基因型的HIV易感性降低了2.77倍，G等位基因受试者的HIV易感性降低了2.72倍。在IL-27 p28 -964A / G和2905T / G（| D'| = 0.942），-964A / G和4603G / A（| D'| = 0.930）之间观察到连锁不平衡（LD）系数。单倍型分析显示，单倍型GGG对MSM人群的HIV感染具有保护作用（OR = 0.313； P <0.001）。总之，调查了深圳HIV阳性人群中HBV和HCV感染的血清阳性率，并探讨了与之相关的危险因素。共感染进行了分析。在HIV单一感染，HIV / HBV合并感染和HIV / HCV合并感染的个体中，血浆IL-27滴度显着升高。 IL-27水平与HIV单一感染人群中CD4 + T细胞计数相关。在HIV / HCV合并感染的个体中，HIV病毒载量与IL-27滴度之间存在显着正相关（r = 0.362，P = 0.049）。 IL-27 p28 2905T / G与个体对HIV感染的易感性有关，单倍型GGG在限制MSM人群的HIV感染中显示出保护作用。

著录项

  • 作者

    He, Lai.;

  • 作者单位

    The Chinese University of Hong Kong (Hong Kong).;

  • 授予单位 The Chinese University of Hong Kong (Hong Kong).;
  • 学科 Health Sciences Public Health.;Health Sciences Epidemiology.;Health Sciences Immunology.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 175 p.
  • 总页数 175
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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