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首页> 外文学位 >Functional analysis of LEAFY COTYLEDON1 during embryo development in Arabidopsis thaliana.
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Functional analysis of LEAFY COTYLEDON1 during embryo development in Arabidopsis thaliana.

机译：LEAFY COTYLEDON1在拟南芥胚胎发育过程中的功能分析。

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摘要

Embryogenesis in higher plants is a critical period during which the basic body plan of plants is established. A single-celled zygote undergoes a series of proliferation and differentiation events to produce a mature embryo that exhibits shoot-root body pattern and all of the primary tissue types. This process is highly regulated by the orchestrated actions of many genes, although the whole regulatory network has not been elucidated. Arabidopsis LEAFY COTYLEDON1 (LEC1) is an important regulator of embryogenesis that controls various aspects of embryo development from the early morphogenesis phase to the late maturation phase and that has the ability to induce the embryonic pathway in vegetative cells. LEC1 encodes an Arabidopsis homolog of the HAP3 subunit of CCAAT binding factor (CBF). CBFs are evolutionarily conserved transcription factor complexes consisting of at least three subunits. Although a functional plant CBF has not yet been demonstrated, Arabidopsis possesses gene families for three subunits, called AHAP2 (Arabidopsis HAP2), AHAP3, and AHAP5. AHAP3 subunits can be divided into functionally distinct two classes, the LEC1-type and the non-LEC1-type, and LEC1 represents the LEC1-type AHAP3 subunits that specifically function during embryogenesis. In this dissertation, I studied mechanisms underlying the functional specificity of LEC1. By analyzing the ability of mutated LEC1 proteins to suppress genetically the lec1 mutation, I showed that the B domain of LEC1 is important for its specific function, and identified one specific amino acid residue within the LEC1 B domain, Asp-55, that is critical for LEC1's activity during embryo development. To obtain insight about how LEC1 functions specifically as a subunit of the CBF complex, I showed that LEC1 forms a ternary protein complex with AHAP2 and AHAP5 subunits in yeast. I also showed that the LEC1-CBF complex constructed in yeast does not possess intrinsic transcriptional activity and does not bind to a yeast CCAAT element. Based on the results, possible mechanisms by which a LEC1-CBF complex specifically regulates the genes required for embryogenesis and the roles of the Asp-55 residue are discussed.
机译：高等植物的胚胎发生是建立植物基本身体计划的关键时期。单细胞合子经历一系列增殖和分化事件，以产生成熟的胚，该胚表现出芽根体模式和所有主要组织类型。尽管尚未阐明整个调控网络，但该过程受到许多基因的协调调控。拟南芥LEAFY COTYLEDON1（LEC1）是重要的胚胎发生调节剂，可控制从早期形态发生阶段到成熟后期的胚胎发育的各个方面，并具有诱导营养细胞中胚胎途径的能力。 LEC1编码CCAAT结合因子（CBF）的HAP3亚基的拟南芥同源物。 CBF是进化保守的转录因子复合物，由至少三个亚基组成。尽管尚未证明功能性植物CBF，但拟南芥拥有三个亚基的基因家族，分别称为AHAP2（拟南芥HAP2），AHAP3和AHAP5。 AHAP3亚基可分为功能不同的两类，即LEC1型和非LEC1型，LEC1代表在胚胎发生过程中特异发挥作用的LEC1型AHAP3亚基。在这篇论文中，我研究了LEC1功能特异性的潜在机制。通过分析突变的LEC1蛋白从基因上抑制lec1突变的能力，我发现LEC1的B结构域对其特定功能很重要，并确定了LEC1 B结构域中的一个特定氨基酸残基Asp-55，这一点至关重要LEC1在胚胎发育过程中的活性。为了了解LEC1如何专门作为CBF复合物的亚基发挥作用，我展示了LEC1与酵母中的AHAP2和AHAP5亚基形成三元蛋白复合物。我还表明，在酵母中构建的LEC1-CBF复合物不具有固有的转录活性，并且不与酵母CCAAT元件结合。基于结果，讨论了LEC1-CBF复合物特异性调节胚胎发生所需基因和Asp-55残基作用的可能机制。

著录项

  • 作者

    Lee, HyeSeung.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Molecular biology.
  • 学位 Ph.D.
  • 年度 2004
  • 页码 86 p.
  • 总页数 86
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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