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Multiple roles of AIP6 in developmental regulation of Arabidopsis.

机译：AIP6在拟南芥发育调控中的多重作用。

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摘要

Genetic studies have implicated the abscisic acid (ABA) transducing factors ABI3, ABI4 and ABI5, in responses to high sugar-induced stress and ABA. Although ABI3 mRNA and protein expression are confined mainly to late embryogenesis, analyses of abi3 mutants indicate the gene functions also during vegetative development. Using the yeast two-hybrid system we identified ABI3 Interacting Protein 6 (AIP6, At3g05545) as a possible regulator of ABI3 function. AIP6 is a RING-type, E3 ubiquitin protein ligase with demonstrated E3 activity in vitro . Our lab has shown that epitope-tagged, full-length AIP6 and ABI3 form a complex in vivo, in yeast and in planta (L. Host et al, 2007). In order to define the function of AIP6 in planta, homozygous aip6 mutant alleles (T-DNA insertion) were characterized. Homozygous aip6 alleles show an increase in seed dormancy and sensitivity to inhibition of germination by ABA, delayed flowering time under short day conditions but not under long day conditions, and an increase in ABI3 protein levels when compared to the wild-type (Col-O) control. All these traits are consistent with enhanced activity of ABI3 and possibly an ABI3-related factor, indicative of a role for AIP6 as a negative regulator of ABI3. Massively Parallel Signature Sequencing (MPSS) and Tilling array demonstrates that AIP6 is expressed ubiquitously in Arabidopsis suggesting multiple targets and possibly multiple roles during plant development.
机译：遗传学研究表明脱落酸（ABA）的转导因子ABI3，ABI4和ABI5响应于高糖诱导的应激和ABA。尽管ABI3 mRNA和蛋白表达主要限于后期胚胎发生，但对abi3突变体的分析表明该基因在营养发育过程中也起作用。使用酵母双杂交系统，我们确定了ABI3相互作用蛋白6（AIP6，At3g05545）作为ABI3功能的可能调节剂。 AIP6是一种RING型E3泛素蛋白连接酶，在体外具有E3活性。我们的实验室表明，带有表位标记的全长AIP6和ABI3在体内，酵母和植物体内形成复合物（L. Host等，2007）。为了定义AIP6在植物中的功能，对纯合aip6突变等位基因（T-DNA插入）进行了表征。与野生型相比，纯合aip6等位基因显示出种子休眠和对ABA抑制发芽的敏感性增加，在短日条件下但不在长日条件下延迟开花时间以及ABI3蛋白水平增加（Col-O ） 控制。所有这些特征与增强的ABI3活性以及可能与ABI3相关的因子一致，表明AIP6作为ABI3的负调节剂。大规模平行签名测序（MPSS）和耕作阵列表明，AIP6在拟南芥中广泛表达，表明在植物发育过程中有多个靶标和可能的多个作用。

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  • 作者

    Jagdeo, Malini Mahadai.;

  • 作者单位

    University of Maryland, Baltimore County.;

  • 授予单位 University of Maryland, Baltimore County.;
  • 学科 Biology Molecular.;Biology Genetics.
  • 学位 Ph.D.
  • 年度 2011
  • 页码 216 p.
  • 总页数 216
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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