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首页> 外文学位 >Evaluation of serum insulin-like growth factor binding proteins and the insulin-like growth factor binding protein-2 locus for potential associations with growth, carcass merit and meat quality in beef cattle.
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Evaluation of serum insulin-like growth factor binding proteins and the insulin-like growth factor binding protein-2 locus for potential associations with growth, carcass merit and meat quality in beef cattle.

机译：评价血清胰岛素样生长因子结合蛋白和胰岛素样生长因子结合蛋白2位点与肉牛生长，屠体价值和肉质的潜在关联。

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摘要

The presence of growth-promoting factors in serum and tissues has been recognized since the beginning of the last century. Recent advances in protein chemistry and the development of molecular biology techniques have resulted in the identification, at both the gene and protein level, of several biological activities suspected to belong to the insulin-like growth factor (IGF) family. The IGFs (IGF-I and -II) are unique among peptide growth factors in that they circulate in blood tightly bound to a group of proteins known as the insulin-like growth factor binding proteins (IGFBP), which modulate IGF activity. The objectives of this study were to evaluate relative abundance of serum IGFBP in Angus cattle divergently selected (high vs. low) for serum IGF-I concentration, to identify DNA sequence variation (polymorphisms) at the bovine IGFBP-2 locus, and to investigate potential associations between IGFBP and traits of economic importance. No significant effect of line was observed in the expression of specific IGFBP species in periods before and after the selection lines were divergent for serum IGF-I concentration. However, heifer calves consistently expressed higher levels of the 34kDa IGFBP species than bulls. This protein was determined to be bovine IGFBP-2 by immunoblot analysis. When the lines were divergent for serum IGF-I, IGFBP-2 was negatively correlated with serum IGF-I concentration and body weights measured throughout the postweaning performance test. Also, two restriction fragment length polymorphisms (RFLP) were identified at the IGFBP-2 locus and both were found to be segregating in different breeds of cattle.{09}IGFBP-2 RFLP alleles identified with the restriction endonuclease Hind III were found to be associated with growth and carcass traits. Results of this study support IGFBP-2 as an important regulator of somatic growth in cattle and further investigation of this gene is warranted.
机译：自上世纪初以来，已经认识到血清和组织中存在促进生长的因子。蛋白质化学的最新进展和分子生物学技术的发展已导致在基因和蛋白质水平上鉴定出怀疑属于胰岛素样生长因子（IGF）家族的几种生物活性。 IGF（IGF-I和-II）在肽生长因子中是独特的，因为它们在血液中紧密结合与一组蛋白结合，这些蛋白被称为胰岛素样生长因子结合蛋白（IGFBP），可调节IGF活性。这项研究的目的是评估以血清IGF-I浓度不同选择（高或低）的安格斯牛的血清IGFBP的相对丰度，以鉴定牛IGFBP-2位点的DNA序列变异（多态性），并调查IGFBP与经济重要性特征之间的潜在关联。在选择系针对血清IGF-I浓度发散之前和之后，在特定IGFBP种类的表达中未观察到该系的显着影响。但是，小母牛犊牛始终表达出比公牛更高的34kDa IGFBP种类。通过免疫印迹分析确定该蛋白为牛IGFBP-2。当品系的血清IGF-1发生分歧时，IGFBP-2与整个断奶后性能测试中测得的血清IGF-1浓度和体重呈负相关。此外，在IGFBP-2位点鉴定出两个限制性片段长度多态性（RFLP），并且发现它们都在不同的牛种中隔离。{09} IGFBP-2 RFLP等位基因以限制性内切酶 Hind鉴定。斜体> III被发现与生长和car体性状有关。这项研究的结果支持IGFBP-2作为牛体细胞生长的重要调节剂，因此有必要对该基因进行进一步的研究。

著录项

  • 作者

    Pagan, Melvin.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Animal Pathology.; Biology Molecular.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 p.2120
  • 总页数 145
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物医学（兽医学）;
  • 关键词

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