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首页> 外文学位 >Regulation of gene expression in the desiccation-tolerant moss Syntrichia ruralis: A study using the early light inducible protein-like genes ELIPa and ELIPb, and the ribosomal protein gene RPL15.
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Regulation of gene expression in the desiccation-tolerant moss Syntrichia ruralis: A study using the early light inducible protein-like genes ELIPa and ELIPb, and the ribosomal protein gene RPL15.

机译：耐旱苔藓Syntrichia Ruralis中基因表达的调节：一项使用早期光诱导蛋白样基因ELIPa和ELIPb以及核糖体蛋白基因RPL15的研究。

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摘要

Syntrichia ruralis (Hedw.) F. Weber & D. Mohr ( Tortula ruralis) is a useful model system for the study of gene control in response to severe water deficit-stress. Two truncated cDNAs (designated Elipa and Elipb) were isolated with significant similarity to the early light-inducible proteins (ELIPs). ELIPs are nuclear encoded, chloroplast thylakoid membrane-associated proteins and were first discovered during early greening of etiolated plants. ELIPs respond to a variety of stress factors, including high light and desiccation, and are postulated to protect the chloroplast from photooxidative damage. Full-length cDNAs of Elipa and Elipb were obtained by 5′ RACE and RLM-RACE respectively. Elipa is 1006 bp in length, encoding a predicted peptide with 212 amino acids (23.3 kDa, pI 5.57). Elipb is 997 bp in length, encoding a predicted peptide with 224 amino acids (24.4 kDa, pI 9.27). ELIPa and ELIPb are most similar to each other, but ELIPa has more similarity to ELIPs from other species. ELIPa contains three transmembrane helices and two potential destruction-liable PEST sequences while ELIPb lacks these motifs entirely. RNA blot hybridization was used to analyze Elipa and Elipb mRNA abundance in response to a variety of stresses. Elipa is a more abundant message than Elipb. Elipa was detectable in all treatments including control and dark treatment. Elipa mRNA was increased in abundance by slow desiccation, rapid dry/rehydration, rehydration in high light, low temperature, high salt, ABA, jasmonic acid and UV-C. Elipb RNA was only detectable in S. ruralis gametophyte subjected to rehydration in high light and ABA treatment.;In addition, a truncated cDNA was isolated with significant similarity to the large-subunit ribosomal protein L15 (RPL15). The full-length cDNA of Rpl15 was obtained using 5′RACE. Rpl15 encodes a predicted peptide with 203 amino acids (24.1 kDa, pI 11.1). RNA blot hybridization demonstrated that Rpl15 mRNA abundance does not change in S. ruralis during a desiccation-rehydration cycle and that Rpl15 transcripts are maintained in desiccated gametophytes as conserved mRNAs.
机译：Syntrichia Ruralis（Hedw。）F. Weber＆D. Mohr（Tortula Ruralis）是研究严重缺水胁迫下基因控制的有用模型系统。分离了两个截短的cDNA（分别称为Elipa和Elipb），它们与早期的光诱导蛋白（ELIPs）具有显着的相似性。 ELIP是核编码的叶绿体类囊体膜相关蛋白，最早是在黄化植物的早期绿化过程中发现的。 ELIP对多种压力因素做出响应，包括强光和干燥，并被认为可以保护叶绿体免受光氧化损伤。通过5'RACE和RLM-RACE分别获得Elipa和Elipb的全长cDNA。 Elipa的长度为1006 bp，编码具有212个氨基酸（23.3 kDa，pI 5.57）的预测肽。 Elipb的长度为997 bp，编码具有224个氨基酸（24.4 kDa，pI 9.27）的预测肽。 ELIPa和ELIPb彼此最相似，但ELIPa与其他物种的ELIP相似度更高。 ELIPb包含三个跨膜螺旋和两个潜在的具有破坏力的PEST序列，而ELIPb则完全缺少这些基序。 RNA印迹杂交用于分析Elipa和Elipb mRNA对多种压力的响应。 Elipa比Elipb更丰富。在包括对照和深色处理在内的所有处理中均可检测到伊利帕。通过缓慢的干燥，快速的干燥/补液，高光，低温，高盐，ABA，茉莉酸和UV-C补液，Elipa mRNA大量增加。 Elipb RNA仅在经过强光复水和ABA处理的农村链球菌配子体中才能检测到。此外，分离出的截短cDNA与大亚基核糖体蛋白L15（RPL15）具有显着相似性。使用5'RACE获得Rpl15的全长cDNA。 Rp15编码具有203个氨基酸（24.1kDa，pI 11.1）的预测的肽。 RNA印迹杂交表明，在脱水复水周期中，S。Ruralis中Rpl15 mRNA的丰度没有变化，Rpl15转录本作为保守的mRNA在干燥的配子体中得以维持。

著录项

  • 作者

    Zeng, Qin.;

  • 作者单位

    Southern Illinois University at Carbondale.;

  • 授予单位 Southern Illinois University at Carbondale.;
  • 学科 Biology Molecular.;Biology Plant Physiology.
  • 学位 Ph.D.
  • 年度 2001
  • 页码 191 p.
  • 总页数 191
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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