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首页> 外文学位 >Identification of molecular markers linked to root penetration ability in rice (Oryza sativa L.).
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Identification of molecular markers linked to root penetration ability in rice (Oryza sativa L.).

机译：鉴定与水稻（Oryza sativa L.）的根部渗透能力有关的分子标记。

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摘要

The main objective of this study is to identify molecular marker like restriction fragment length polymorphism (RFLP) associated with penetrated root number, penetrated root thickness and root penetration ability of indica rice through wax-petrolatum layer simulated as compacted soil layer. Mapping population of this study composed of 166 recombinant inbred lines (RILs) developed from two indica genotypes was evaluated in the greenhouse twice with five replications. Data were collected for total root number (TRN), penetrated root number (PRN), root penetration index (RPI), and penetrated root thickness (PRT). The TRN varied from 71-328 with a mean of 161 and 45-213 with a mean of 98 in summer and fall experiments, respectively. The PRN varied from 14-122 with a mean of 40 and 6-57 with a mean of 21 in summer and fall experiments, respectively. The RPI varied from 0.08-0.56 with a mean of 0.26 in summer and 0.06-0.55 with a mean of 0.23 in fall experiment. The PRT varied from 0.40-1.18 mm with a mean of 0.82 mm and 0.40-1.10 mm with a mean of 0.78 mm in summer and fall experiments, respectively. A total of 16 QTLs were detected by single-point and interval mapping analysis in two experiments; three QTLs for TRN, four QTLs for PRN, four QTLs for RPI and five QTLs for PRT. Seven QTLs were found common in both the experiments, of which three QTLs for PRN explained total phenotypic variation of 43%, one QTL for RPI explained 22% phenotypic variation, and three QTLs for PRT explained together 26% of the phenotypic variation. One QTL detected in the interval between markers RG151 and RG256 was found to influence penetrated root number, root penetration index and penetrated root thickness. Another QTL flanked by the markers AA7-2a and AA7-2b influenced both penetrated root number and root penetration index. These two QTLs were found to be located in the same chromosomal regions of other populations with different genetic backgrounds. Markers which are tightly linked with the detected QTLs for root penetration ability and penetrated root thickness will facilitate the transfer of these QTLs to susceptible indica cultivars to improve drought resistance employing the marker-aided selection approach. (Abstract shortened by UMI.).
机译：这项研究的主要目的是鉴定与marker稻相似的分子标记，如限制性片段长度多态性（RFLP），其通过模拟为压实的土壤层的蜡-凡士林层与penetrate稻的穿透根数，穿透根厚和根系穿透能力有关。在温室中两次评估了由两个two型基因型组成的166个重组自交系（RIL）组成的本研究的作图群体。收集总根数（TRN），穿透根数（PRN），根部穿透指数（RPI）和穿透根部厚度（PRT）的数据。在夏季和秋季实验中，TRN的平均值分别为71-328和161，而45-213平均值为98。在夏季和秋季实验中，PRN分别从14-122（平均值为40）和6-57（平均值为21）变化。夏季实验的RPI在0.08-0.56之间变化，平均值为0.26，在秋季实验中为0.06-0.55，平均值为0.23。在夏季和秋季实验中，PRT分别为0.40-1.18毫米（平均值为0.82毫米）和0.40-1.10毫米（平均值为0.78毫米）。通过两个实验中的单点和间隔作图分析，总共检测到16个QTL。 TRN的三个QTL，PRN的四个QTL，RPI的四个QTL和PRT的五个QTL。在两个实验中发现共有七个QTL，其中三个PRN QTL解释了总表型变异43％，一个RPI QPI解释了22％的表型变异，三个PRT QTL解释了26％的表型变异。发现在标记RG151和RG256之间的间隔中检测到的一个QTL影响根系穿透根数，根系穿透指数和根系穿透厚度。标记为AA7-2a和AA7-2b的另一个QTL影响穿透根数和根穿透指数。发现这两个QTL位于具有不同遗传背景的其他人群的相同染色体区域中。与检测到的QTL紧密结合的根部穿透能力和根部穿透厚度的标记，将有助于使用标记辅助选择方法将这些QTL转移到易感in稻品种上，从而提高抗旱性。 （摘要由UMI缩短。）。

著录项

  • 作者

    Pathan, Md. Safiullah.;

  • 作者单位

    Texas Tech University.;

  • 授予单位 Texas Tech University.;
  • 学科 Agriculture Agronomy.;Biology Genetics.
  • 学位 Ph.D.
  • 年度 1998
  • 页码 76 p.
  • 总页数 76
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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