• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Isolation and characterization of Anoxybacillus from alkaline hot springs and thermal soils of Yellowstone National Park.
【24h】

Isolation and characterization of Anoxybacillus from alkaline hot springs and thermal soils of Yellowstone National Park.

机译：黄石国家公园碱性温泉和热土中厌氧杆菌的分离和鉴定。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many thermophilic bacteria have now been identified. Only a few of these bacteria are alkalithermophiles, which grow at temperatures above 50°C and at pH levels above 8.0 In an effort to identify and characterize additional alkalithermophilic bacteria, 25 samples were collected from 6 different alkaline hot springs and thermally heated areas within Yellowstone National Park (YNP). Microbial isolates were obtained by streak and enrichment cultures on different selective media. Five of the six sampling sites yielded alkalithermophilic bacteria. Phylogenetic analysis of 16S rDNA indicated that the majority of these isolates were members of the genus Anoxybacillus. On the basis of morphology and biochemical characteristics, one of the isolates, designated B-25 was further characterized. B-25 is a gram positive, sporulating, rod-shaped bacterium. B-25 can utilize a wide range of nutrient sources, including glucose, maltose, sucrose, and starch, as well as grow on both dilute and high concentrations of organic media. Isolate B-25's optimal growth conditions were determined to be at a pH of 9.0 and at a temperature of 60°C. Isolate B-25 had a very high growth rate (20 minute generation time) and sporulates with a high efficiency. It is highly susceptible to a number of commercial antibiotics and produces the following enzymes: amylase, catalase, and oxidase. 16S rDNA gene sequence analysis on B-25 showed that it is most closely related to Anoxybacillus kualawohkensis and Anoxybacillus kamchatkensis which were recently isolated from hot springs in Malaysia and Russia respectively. Our results suggest that Isolate B-25 is a new species of Anoxybacillus.
机译：现在已经鉴定出许多嗜热细菌。这些细菌中只有少数是碱热菌，它们会在高于50°C的温度和pH值高于8.0的条件下生长。为了鉴定和表征其他的碱热菌，从6个不同的碱性温泉和黄石公园内的热加热区域收集了25个样品国家公园（YNP）。通过在不同的选择培养基上进行条纹和富集培养获得微生物分离株。六个采样点中有五个产生了嗜碱细菌。 16S rDNA的系统发育分析表明，这些分离物中的大多数是无氧杆菌属的成员。根据形态和生化特性，进一步鉴定了一种分离株，命名为B-25。 B-25是革兰氏阳性，呈孢子状的杆状细菌。 B-25可以利用各种营养素，包括葡萄糖，麦芽糖，蔗糖和淀粉，也可以在稀和高浓度的有机培养基上生长。确定菌株B-25的最佳生长条件为pH值为9.0，温度为60°C。分离物B-25具有非常高的生长速度（20分钟的生成时间），并具有很高的孢子形成效率。它对多种商业抗生素高度敏感，并产生以下酶：淀粉酶，过氧化氢酶和氧化酶。 B-25的16S rDNA基因序列分析表明，它与最近从马来西亚和俄罗斯的温泉中分离出的kualawohkensis嗜热芽孢杆菌和kamchatkensis嗜热芽孢杆菌密切相关。我们的结果表明，分离株B-25是一种新的厌氧杆菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号