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首页> 外文学位 >An evaluation of methicillin resistant Staphylococcus aureus survival on five environmental surfaces under two different humidities, with and without the addition of bovine serum albumin.
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An evaluation of methicillin resistant Staphylococcus aureus survival on five environmental surfaces under two different humidities, with and without the addition of bovine serum albumin.

机译：在添加和不添加牛血清白蛋白的情况下，评估了在两种不同湿度下五个环境表面上耐甲氧西林的金黄色葡萄球菌的存活率。

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摘要

The spread of antibiotic resistant bacteria is a major public health concern, as they result in greater healthcare costs and increased morbidity and mortality rates. Methicillin resistant Staphylococcus aureus (MRSA) is one organism of particular concern, with the number of infections increasing in epidemic proportion. Bacterial surface contamination with MRSA is significant, as it may serve as a reservoir for transmission and have negative health implications. The purpose of this study was to evaluate survival of MRSA on five environmental surface materials; glass, wood, vinyl, plastic, and cloth. The effect of relative humidity (RH) and bovine serum albumin (BSA) were also examined. Surfaces of 5.1cm2 were inoculated with 3.0 x 108 -- 1.4 x 109 MRSA CFU/ml with and without 1% BSA. Surfaces were incubated at 35°C at 45--55% and 16% RH. Hard surfaces were swab sampled and cloth surfaces hand stomached and re-suspended in phosphate buffer (PB). Suspensions of 100microl were spread plated onto agar plates and incubated at 35°C for 24 hrs; resulting colonies were enumerated. Samples were taken immediately upon drying (time 0), 3 hrs, 24 hrs, 2 days, 3 days, 4 days, and 5 days. Results showed that there was a significant difference (p.001) among the surfaces; MRSA survived on plastic and vinyl for the longest amount of time, and wood for the least amount of time. The overall greatest concentration of CFU/ml remained on cloth; however, the MRSA did not persist past 3 days. The addition of BSA enabled MRSA to survive significantly longer (p.001). The amount of CFU/ml was significantly less (p=.002) on surfaces stored in 45--55% RH versus 16% RH. Information gathered from this study indicates that viable MRSA bacteria can remain on surfaces for days; likely serving as a reservoir for transmission. The best way to control the MRSA epidemic is to prevent transmission.
机译：抗生素抗性细菌的扩散是主要的公共卫生问题，因为它们导致更高的医疗费用以及更高的发病率和死亡率。耐甲氧西林的金黄色葡萄球菌（MRSA）是一种特别值得关注的生物，其感染数量在流行中呈上升趋势。 MRSA对细菌表面的污染非常重要，因为它可能充当传播的储存库，并对健康产生负面影响。这项研究的目的是评估MRSA在五种环境表面材料上的存活率。玻璃，木材，乙烯基，塑料和布料。还检查了相对湿度（RH）和牛血清白蛋白（BSA）的影响。在有和没有1％BSA的情况下，用3.0 x 108-1.4 x 109 MRSA CFU / ml接种5.1平方厘米的表面。将表面在35°C，45--55％和16％RH的条件下孵育。取样硬表面，并用手将布表面拿起并重新悬浮在磷酸盐缓冲液（PB）中。将100μl的悬浮液铺展在琼脂平板上，并在35℃下孵育24小时。计数得到的菌落。在干燥（时间0），3小时，24小时，2天，3天，4天和5天时立即取样。结果表明，表面之间存在显着差异（p <.001）。 MRSA在塑料和乙烯基上的生存时间最长，而在木材上的生存时间最少。总的最大CFU / ml浓度仍留在布上。但是，MRSA并没有持续超过3天。添加BSA可使MRSA存活更长的时间（p <.001）。与16％RH相比，在45--55％RH中存储的表面上的CFU / ml量明显更少（p = .002）。从这项研究中收集到的信息表明，有活力的MRSA细菌可以在表面停留数天。可能充当传播的储存库。控制MRSA流行病的最佳方法是防止传播。

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  • 作者

    Coughenour, Courtney Ann.;

  • 作者单位

    University of Nevada, Las Vegas.;

  • 授予单位 University of Nevada, Las Vegas.;
  • 学科 Health Sciences Occupational Health and Safety.;Health Sciences Public Health.;Biology Microbiology.
  • 学位 M.P.H.
  • 年度 2009
  • 页码 49 p.
  • 总页数 49
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
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