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首页> 外文学位 >RNA POLYMERASE III TRANSCRIPTION IN THE SILKWORM: IDENTIFICATION OF TRANSCRIPTIONAL SIGNALS IN TWO TYPES OF GENES (RETROPOSON, REPETITIVE SEQUENCES).
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RNA POLYMERASE III TRANSCRIPTION IN THE SILKWORM: IDENTIFICATION OF TRANSCRIPTIONAL SIGNALS IN TWO TYPES OF GENES (RETROPOSON, REPETITIVE SEQUENCES).

机译：丝绸中的RNA聚合酶III转录：两种基因（转录子，重复序列）中转录信号的鉴定。

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摘要

Cis-acting sequences can act as important signals affecting the expression of genes in eukaryotes. I have analyzed DNA sequence signals of two types of genes from Bombyx mori that are transcribed by RNA polymerase III, the polymerase responsible for the expression of tRNA, 5S RNA, and other small RNA gene sequences.;The tRNA('Ala) control region is large relative to control regions previously identified for tRNA genes in other organisms. To understand this discrepancy, in this work I have studied the effect of variation of assay conditions on the extent of sequences that appear to be important for transcriptional control. These experiments show that certain assay parameters have a determinative effect on the apparent size of the tRNA('Ala) control region; certain conditions can obscure the importance of portions of tRNA('Ala) control sequences. My results suggest that previous transcriptional analysis of tRNA genes may not have identified all sequences that contribute to the success of the reaction.;Second, I have studied the transcription of a previously undescribed gene sequence, BmX, by the silkworm RNA polymerase III. I discovered the BmX template, and have characterized the family of repetitive DNA sequences (20,000 copies/genome) to which it belongs. I have analyzed in detail a member of the BmX gene family, identifying transcriptional initiation and termination sites. To identify the transcriptional control region of the BmX gene, mutant derivatives of the BmX sequence were analyzed in in vitro transcription experiments. Like the tRNA('Ala) gene control region, the BmX control region is large, including 5' flanking, coding, and 3' flanking sequences. BmX gene sequences also bind the limiting transcription factor(s) in silkworm crude extracts.;First, I have characterized sequences that contribute to the expression of a tRNA('Ala) gene in in vitro transcription experiments. The tRNA('Ala) control region defined in this work is very large, including (TURN)30 bp of 5' flanking DNA, coding sequences, and (TURN)50 bp of 3' flanking DNA. A subset of these tRNA('Ala) control sequences are essential for binding a limiting transcription factor(s) found in crude cell extracts.
机译：顺式作用序列可以作为影响真核生物中基因表达的重要信号。我已经分析了家蚕的两种基因的DNA序列信号，这些基因被RNA聚合酶III转录，该聚合酶负责tRNA，5S RNA和其他小RNA基因序列的表达。tRNA（'Ala）控制区相对于先前在其他生物中发现的tRNA基因的对照区域而言，该区域相对较大。为了理解这种差异，在这项工作中，我研究了测定条件变化对似乎对转录控制很重要的序列范围的影响。这些实验表明，某些测定参数对tRNA（'Ala）对照区域的表观大小具有决定性影响。某些条件会掩盖tRNA（'Ala）控制序列部分的重要性。我的结果表明，先前对tRNA基因的转录分析可能尚未鉴定出有助于反应成功的所有序列。其次，我研究了蚕RNA聚合酶III对先前未描述的基因序列BmX的转录。我发现了BmX模板，并对它所属的重复DNA序列家族（20,000个拷贝/基因组）进行了表征。我已经详细分析了BmX基因家族的成员，确定了转录起始和终止位点。为了鉴定BmX基因的转录控制区，在体外转录实验中分析了BmX序列的突变衍生物。像tRNA（'Ala）基因控制区一样，BmX控制区也很大，包括5'侧翼，编码和3'侧翼序列。 BmX基因序列还与家蚕粗提物中的限制性转录因子结合。首先，我对在体外转录实验中有助于tRNA（'Ala）基因表达的序列进行了表征。这项工作中定义的tRNA（'Ala）控制区非常大，包括（TURN）30'的5'侧翼DNA，编码序列和（TURN）50 bp的3'侧翼DNA。这些tRNA（'Ala）控制序列的子集对于结合在粗细胞提取物中发现的限制性转录因子是必不可少的。

著录项

  • 作者

    WILSON, ELLEN TARRANT.;

  • 作者单位

    University of Oregon.;

  • 授予单位 University of Oregon.;
  • 学科 Biology Molecular.
  • 学位 Ph.D.
  • 年度 1986
  • 页码 148 p.
  • 总页数 148
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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