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首页> 外文学位 >ANALYSIS OF ULTRAVIOLET MICROBEAM IRRADIATIONS OF SINGLE CHROMOSOMAL SPINDLE FIBRES IN CRANE FLY SPERMATOCYTES.
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ANALYSIS OF ULTRAVIOLET MICROBEAM IRRADIATIONS OF SINGLE CHROMOSOMAL SPINDLE FIBRES IN CRANE FLY SPERMATOCYTES.

机译：鹤蝇精细胞中单染色体纺锤状纤维的紫外线微束辐照分析。

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摘要

This thesis is comprised of four papers all of which describe the results of ultraviolet (UV) microbeam irradiations of chromosomal spindle fibres in crane fly spermatocytes. Each paper deals with a specific aspect of mitosis, that is, either coordination between chromosomes or the components involved in force production and birefringence of spindle fibres. We irradiated portions of single autosomal spindle fibres and found that these irradiations altered the subsequent sex chromosome movements. In unirradiated meiosis I cells, the 3 autosomal half-bivalents move to each pole in anaphase while the 2 sex chromosomal univalents remain at the equator. The sex chromosomes move to opposite poles only after the autosomes have reached the poles. After irradiations either of 2 effects were observed. One effect was that the sex chromosomes did not move at all and the second effect was that both sex chromosomes moved to the same pole, always that of the non-irradiated side. When single chromosomal spindle fibres in anaphase were irradiated with monochromatic light the movement of the half-bivalent associated with the irradiated spindle fibre stopped moving (if the dose was high enough) as did the partner half-bivalent moving to the opposite pole. All the other half-bivalents moved normally. The action spectrum for stopping chromosome movement was determined, it had two peaks, one at 270nm and the other at 290nm. Action spectra were obtained for blocking ciliary beating and myofibril contraction and compared to the action spectrum for stopping chromosome movement. The action spectrum for changes in spindle fibre birefringence was determined, it had 2 peaks, one at 260nm and the other at 280nm. Indeed the components contributing to force production and those contributing to birefringence have different chromophores in crane fly spermatocytes. The implications of this result to the various hypotheses of chromosome movement is discussed.
机译：本论文由四篇论文组成，所有这些论文描述了鹤蝇精细胞中染色体纺锤体纤维的紫外线（UV）微束辐照的结果。每篇论文都涉及有丝分裂的一个特定方面，即染色体之间的协调或参与力产生和纺锤丝双折射的成分。我们辐照了部分常染色体纺锤体纤维，发现这些辐照改变了随后的性染色体运动。在未辐照的减数分裂I细胞中，3个常染色体半价在后期进入每个极点，而2个性染色体单价仍停留在赤道处。只有在常染色体到达极点后，性染色体才会向相反的方向移动。照射后，观察到两种效果中的任何一种。一种作用是性染色体完全不移动，第二种效果是两性染色体都移动到相同的极点，始终是未辐照的那一侧。当用单色光辐照后期的单个染色体纺锤体纤维时，与辐照纺锤体纤维相关的半二价运动停止移动（如果剂量足够高），而另一半二价运动到另一极。所有其他半价部分均正常移动。确定了用于阻止染色体移动的作用谱，它具有两个峰，一个在270nm，另一个在290nm。获得了用于阻断纤毛跳动和肌原纤维收缩的作用谱，并与用于阻止染色体运动的作用谱进行了比较。确定了纺锤体纤维双折射变化的作用谱，它具有2个峰，一个在260nm，另一个在280nm。确实，促力产生的组分和促双折射的组分在鹤蝇精细胞中具有不同的生色团。讨论了该结果对染色体运动的各种假设的影响。

著录项

  • 作者

    SILLERS, PEGGY JANICE MARGARET.;

  • 作者单位

    York University (Canada).;

  • 授予单位 York University (Canada).;
  • 学科 Biology General.
  • 学位 Ph.D.
  • 年度 1983
  • 页码 164 p.
  • 总页数 164
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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