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In vitro, Non-invasive Imaging and Detection of Single Living Mammalian Cells Interacting with Bio-nano-interfaces.

机译：体外，非侵入性成像和与生物纳米接口相互作用的单个哺乳动物细胞的检测。

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摘要

Understanding of bio-nano-interfaces of living mammalian cells will benefit the identification of cellular alterations (e.g. nucleic acids, amino acids, biomechanics, etc.) due to external stimuli, the design of biomaterials (e.g. nanoparticles, nanotubes) and the investigation of the interaction between cells and bio-nano-interfaces (e.g. cell differentiation on 3D nanostructured materials). Analytical techniques can be applied to evaluate the chemical, physical, and mechanical properties of mammalian cells when exposed to such bio-nano-interfaces. In this study, non-invasive advanced spectroscopy techniques including atomic force microscopy (AFM) and Raman microscopy (RM), in conjunction with traditional biological methods are utilized to elucidate specific characteristic information for biological samples and how these property changes reflect the interaction with external stimuli.;The focus of this dissertation is on the biophysical, biochemical and cytotoxic detection of mammalian cells interacting with bio-nano-interfaces, and this dissertation can be classified into three topics: biomechanics/cellular biopolymers measurement, bio-interfaces and nano-interfaces studies.;For the topic of biomechanics/cellular biopolymers measurement, cellular biophysical and biomechanical properties could be used as differentiation markers to classify cellular differentiation. For the bio-interfaces part, it was observed that BRMS1 expression changed cellular biochemical and biomechanical properties, and the expressions of reactive oxidative species (ROS), apoptosis and cell viability of five types of cells displayed similar patterns over doxorubicin (DOX) incubation time. Secondly, A549 cells were treated with diesel exhaust particles (DEP) and resveratrol (RES) to study the effect of RES on the DEP-induced cells, and it was found that RES can alleviate DEP intervention on cellular structure and increase DEP-induced biomechanical and inflammatory changes. For the nano-interfaces topic, first we synthesized a hybrid nanoparticle with the multimodal properties of fluorescence imaging, Surface-enhanced Raman spectroscopy (SERS) detection and photothermal therapy (PTT) for single living cell analysis of epidermal growth factor receptor (EGFR) and specifically killed cancer cells with high EGFR expression. Additionally, to increase surface area, light-heat conversion efficiency and biocompatibility, we developed a silica coated nanoparticle conjugated with anti-human epidermal growth factor receptor 2 (HER2) antibody. Finally, three-dimensional TiO 2 nanotubes with Au nanoparticles coating were synthesized and used to study trophoblast-derived stem-like cells growth on such 3D nanostructures.
机译：了解活体哺乳动物细胞的生物-纳米界面将有助于识别由于外部刺激而引起的细胞变化（例如核酸，氨基酸，生物力学等），生物材料的设计（例如纳米粒子，纳米管）以及对纳米粒子的研究。细胞与生物纳米界面之间的相互作用（例如3D纳米结构材料上的细胞分化）。当暴露于这种生物-纳米界面时，分析技术可用于评估哺乳动物细胞的化学，物理和机械性能。在这项研究中，包括原子力显微镜（AFM）和拉曼显微镜（RM）在内的非侵入式高级光谱技术与传统生物学方法结合使用，阐明了生物样品的特定特征信息，以及这些特性的变化如何反映与外界的相互作用。论文的重点是与生物纳米界面相互作用的哺乳动物细胞的生物物理，生化和细胞毒性检测，该论文可分为三个主题：生物力学/细胞生物聚合物测量，生物界面和纳米技术。界面研究。；对于生物力学/细胞生物聚合物测量的主题，细胞生物物理和生物力学特性可以用作区分细胞分化的标记。对于生物界面部分，观察到BRMS1表达改变了细胞的生化和生物力学特性，并且五种类型的细胞的反应性氧化物质（ROS），细胞凋亡和细胞活力的表达在阿霉素（DOX）孵育时间内表现出相似的模式。 。其次，用柴油机尾气颗粒（DEP）和白藜芦醇（RES）处理A549细胞，研究RES对DEP诱导的细胞的作用，发现RES可以减轻DEP对细胞结构的干预，增加DEP诱导的生物力学。和炎症变化。对于纳米界面主题，我们首先合成了具有荧光成像，表面增强拉曼光谱（SERS）检测和光热疗法（PTT）的多峰特性的杂化纳米颗粒，用于对表皮生长因子受体（EGFR）和特异性杀死具有高EGFR表达的癌细胞。此外，为了增加表面积，光热转换效率和生物相容性，我们开发了与抗人表皮生长因子受体2（HER2）抗体缀合的二氧化硅涂层纳米颗粒。最后，合成了具有金纳米颗粒涂层的三维TiO 2纳米管，并用于研究滋养细胞衍生的干样细胞在此类3D纳米结构上的生长。

著录项

  • 作者

    Li, Qifei.;

  • 作者单位

    Utah State University.;

  • 授予单位 Utah State University.;
  • 学科 Biomedical engineering.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 251 p.
  • 总页数 251
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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