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首页> 外文学位 >Base-resolution sequencing method for 5-formylcytosine and 5-carboxylcytosine and genome-wide mapping methods for 5-hydroxymethylcytosine from low input DNA
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Base-resolution sequencing method for 5-formylcytosine and 5-carboxylcytosine and genome-wide mapping methods for 5-hydroxymethylcytosine from low input DNA

机译：5-甲酰基胞嘧啶和5-羧基胞嘧啶的基本分辨率测序方法和低输入DNA的5-羟甲基胞嘧啶全基因组定位方法

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摘要

Recently, new DNA modifications have been discovered in the mammalian genome, namely 5-hydroxymethylcytosine (5hmC), 5-formylcysotine (5fC) and 5-carboxylcytosine (5caC), which have broad impact in biological functions. We have been working on developing effective sequencing technologies to reveal the functional roles of these newly discovered DNA modifications in biological samples by using selective chemical labeling and low input DNA library construction technologies. We have developed chemical assisted bisulfite sequencing (CAB-Seq) for base-resolution sequencing of 5fC and 5caC, DNA immunoprecipitation-coupled chemical-modification assisted bisulfite sequencing (DIP-CAB-Seq) for genome-wide base-resolution sequencing of 5fC and 5caC, 5hmC selective chemical labeling from low input DNA (nano- Seal) for 5hmC profiling and Tet-assisted bisulfite sequencing from low input DNA (nano-TAB-Seq) for base-resolution sequencing of 5hmC. By applying these methods, we have studied the accurate distribution of these new mammalian DNA modifications and are able to study their functions in varies biological pathways and human disease. Together our studies add to a more complete picture of the dynamic epigenetic regulatory network that plays critical roles in human development and disease.
机译：最近，在哺乳动物基因组中发现了新的DNA修饰，即5-羟甲基胞嘧啶（5hmC），5-甲酰基胱氨酸（5fC）和5-羧胞嘧啶（5caC），它们对生物学功能具有广泛的影响。我们一直在努力开发有效的测序技术，以通过使用选择性化学标记和低投入DNA库构建技术来揭示这些新发现的DNA修饰在生物样品中的功能作用。我们已经开发了用于5fC和5caC碱基分辨率测序的化学辅助亚硫酸氢盐测序（CAB-Seq），用于5fC和5fC基因组范围全基因组测序的DNA免疫沉淀偶联化学修饰辅助亚硫酸氢盐测序（DIP-CAB-Seq）从低输入DNA进行5caC，5hmC选择性化学标记（nano-Seal）进行5hmC分析，从低输入DNA进行Tet辅助亚硫酸氢盐测序（nano-TAB-Seq）进行5hmC的碱基分辨率测序。通过应用这些方法，我们研究了这些新的哺乳动物DNA修饰的准确分布，并能够研究它们在各种生物学途径和人类疾病中的功能。我们的研究共同为动态表观遗传调控网络增添了更完整的图景，后者在人类发展和疾病中起着关键作用。

著录项

  • 作者

    Lu, Xingyu.;

  • 作者单位

    The University of Chicago.;

  • 授予单位 The University of Chicago.;
  • 学科 Biochemistry.;Bioinformatics.
  • 学位 Ph.D.
  • 年度 2015
  • 页码 98 p.
  • 总页数 98
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 宗教;
  • 关键词

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