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Fungal oil with EPA in Mucor circinelloides cultivated on thin stillage from corn-toethanol production.

机译：玉米（Mucor circinelloides）中的EPA真菌油在玉米酒和乙醇生产的稀酒中培养。

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摘要

This study demonstrates the potential of the oleaginous fungus Mucor circinelloides for synthesizing oil rich in polyunsaturated fatty acids when cultivated in thin stillage (TS) and centrifuged thin stillage (CTS) from corn-to-ethanol production. Biomass yield, oil yield and its EPA content were studied at different cultivation periods and carbon to nitrogen ratios. The fungal biomass yield (g/L) and its oil content (g/L) were found to be twice as high in TS as in CTS when harvested after two to six days. EPA content doubled in TS and increased by 2.6 times in CTS when the cultivation period was extended from three to six days. Glycerol supplementation at 20g/L of C led to an 18% increase and 63.6% decrease in fungal oil yield in TS and CTS respectively. Glycerol addition also increased EPA content by 33% in TS and 60% in CTS. Adding 5g/L N using ammonium sulfate as a nitrogen source enhanced the fungal oil yield by 34% in TS and 26% in CTS and EPA content by 32% in TS and 12% in CTS. Urea at 0.5 g/L of N increased the EPA content by 22% in TS and 33% in CTS and oil content by 12% in TS and 13% in CTS. Potassium nitrate at 5g/L of N increased EPA content by 28% in TS and 33% in CTS at 0.5 g/L of N dosage and increase in oil yield by 18% in TS and decreased oil yield in CTS by 37%.
机译：这项研究证明了油性真菌Mucor circinelloides的潜在潜力，即在玉米秸秆或乙醇生产的稀釜馏物和离心稀釜馏物（CTS）中进行栽培时，可以合成富含多不饱和脂肪酸的油。在不同的栽培时期和碳氮比下，研究了生物质产量，油脂产量及其EPA含量。发现两到六天后收获的真菌生物量（g / L）和油含量（g / L）在TS中是CTS的两倍。当培养时间从三天延长到六天时，TS中的EPA含量增加了一倍，而CTS中的EPA含量增加了2.6倍。补充20g / L的甘油会导致TS和CTS的真菌油产量分别增加18％和63.6％。甘油的添加还使TS中的EPA含量增加了33％，CTS中的EPA含量增加了60％。使用硫酸铵作为氮源添加5g / L N可使TS中的真菌油收率提高34％，CTS中提高26％，TS中的EPA含量提高32％，CTS中提高12％。氮含量为0.5 g / L的尿素可使TS中的EPA含量增加22％，CTS中的33％，TS中的油含量增加12％，CTS中的13％。 N含量为5g / L时的硝酸钾使氮的添加量为0.5 g / L时，TS中的EPA含量增加28％，CTS中增加33％，TS中的油产量增加18％，CTS中的油产量减少37％。

著录项

  • 作者

    Ravi, Shashank.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Agriculture Food Science and Technology.;Alternative Energy.;Biology Microbiology.;Engineering Environmental.
  • 学位 M.S.
  • 年度 2014
  • 页码 42 p.
  • 总页数 42
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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