首页> 中文期刊> 《西北农业学报》 >陕西及宁夏盐渍土中拮抗放线菌资源研究

陕西及宁夏盐渍土中拮抗放线菌资源研究

         

摘要

以分离自盐渍土中的放线菌作为供试菌株,以养殖业中常见的9种动物病原菌作为靶标菌进行拮抗放线菌研究,并对4株优良拮抗菌株进行初步鉴定.结果表明,供试的1172株放线菌主要以链霉菌属为主,占到88.82%,有拮抗活性的菌株551株,占到47.01%;供试放线菌对不同靶标菌的抑菌作用差异较大,对金黄色球菌和无乳链球菌拮抗效果较好;菌株01F10为白孢类群,与Streptomyces qinglanensis亲缘关系最近,序列相似度为99.11%;菌株02H13和14H03为粉红孢类群,与Streptomyces gobitricini序列相似度达到100%;菌株07F10为灰褐类群,与Streptomyces sparsus亲缘关系最近,序列相似度为99.69%.%Actinomyces stranins were isolated from saline soil and 9 bacteria strains were used as the indicator in this test. 4 antagonistic strains with good antagonistic effect were identified. The results showed: the main sort of the 1172 actinomycetes in these areas is streptomyces. the rate was 88. 82%. And 551 strains showed antagonistic effects. Actinomycetes tested for different target bacteria have different effects, and have better antagonistic effects on Staphyloccocus aureus and bovine Streptococcus agalactiae. 4 excellent antagonistic actinomucetes identification results showed that the strain 01F10 named Albosporus, close related to Streptomyces qinglanensis , the sequence similarity is 99. 11%;The strain 02H13 and 14H03 named Roseosporus, close related to Streptomycesgobitricini, the sequence similarity are 100% ;The strain 07F10 named Griseofuscu, close related to Streptomyces sparsus qinglanensis, the sequence similarity is 99. 69%.

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