RG-8M, early-maturing mutant of late-maturing rape RG-8,was used in the present study. A LEAFY(LFY) homologue gene named BnLFY was cloned from RG-8M with the method of homologous cloning. The full length of BnLFY cDNA was 1 310 bp and contained a complete open reading frame of 1 248 bp,which encoded 415 amino acids residues. As other dicotyledon.LFY-like proteins,the deduced a-mino acid sequence of BnLFY gene had several special regions such as N terminal proline rich region, the central acidic domain,the leucine zipper structure and the lysine and arginine-rich basic region. The result of sequencing indicated that the BnLFY protein shared more than 84% amino acid sequence identity with LFY-like proteins of several cruciferous plants. Transchptional analysis showed the BnLFY gene expressed constitutively in rape.%以甘蓝型油菜晚熟品种RG-8的早熟突变体RG-8M为材料,通过同源克隆法得到1个LEAFY(LFY)同源基因,命名为BnLFY.该基因cDNA全长为1 310 bp,包含1个长为1 248 bp的开放阅读框,编码415个氨基酸.序列分析表明,推测的氨基酸序列含双子叶植物LFY类蛋白特有的N端脯氨酸富集区、中央酸性区、亮氨酸拉链结构以及富含赖氨酸与精氨酸的碱性区；且与几种十字花科植物LFY类蛋白的氨基酸序列一致性均在84％以上.转录表达分析表明,BnLFY基因在油菜中为组成型表达.
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