目的：研究缬草挥发性成分。方法：采用：以《药典（一部）》附录XD甲的标准方法提取缬草挥发性成分，采用GC－MS结合化学计量学方法对缬草挥发性成分进行定性分析。色谱柱：5 HP－MS；载气：He；流速：1．0mL／min；进样量：1μL；分流比：1∶100。质谱条件：电子轰击（ EI）离子源，辅助线温度280℃，离子源温度230℃，四级杆温度150℃；质量扫描范围：m／z 50～550。采用Chemstation和Nist 08谱库检索。结果：共鉴定出30种成分，占总含量的94％。结论：共鉴定出成分，与传统定性分析方法相比，GC－MS结合化学计量学方法具有较高的灵敏度和准确度，有效增加鉴定的成分，可用于缬草挥发性成分的定性分析。%Objective:To analyze the volatile components in Valeriana officinalis L.Methods: The volatile components were extracted by steam-stilling.GC-MS-AMDIS combined with Retention index technology was used for the quali -tative analysis of the volatile components in Valeriana officinalis L, 5HP-MS quartz capillary column (30m ×0.25mm ×0.25μm); temperature programcarrier gas ( He) , velocity 1.0mL/min,the injection volume 1μL;split ratio 1∶100. MS conditions:electron impact ( EI) ion source, transmission line temperature 280 ℃, ion source temperature 230℃, Quadrupole temperature 150 ℃mass scan range m/z 50~550.And the results were contrasted with Chemstation and Nist 05a.Results:Totally, 30 compounds which accounted for 94%of the whole extracts of Valeriana officinalis L were identified.Conclusion:Compared with the traditional qualitative analysis method, GC-MS-AMDIS combined with re-tention index technology has a higher sensitivity and accuracy,and can be used for qualitative analysis of the volatile con-stituents of Valeriana officinalis L.
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