CRISPR/Cas9是一种多功能的基因工程技术,除了基因编辑,在基因表达的调控,活细胞DNA/RNA成像等方面也受到了广泛的关注.然而,CRISPR/Cas9系统源自原核生物,要应用到其他生物体内,还需要有运载体的协助.本文结合CRISPR/Cas9技术在多方面的应用,对其运载体进行了综述,为相关领域的工作提供参考.%CRISPR/Cas9 is a versatile gene engineering technology that has been received extensive attention in gene editing, regulation of gene expression and live cell DNA/RNA imaging as well. However, the CRISPR/Cas9 system is derived from prokaryotes, which requires delivery vehicles for application in other organisms. Combined with a variety of applications of CRISPR/Cas9 technology, this paper reviews its delivery vehicles to providing a reference to the work of relevant fields.
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