OBJECTIVE: Through researching of the TGFB1 SNP of Han population in Changsha area, the relationship between the TGFBl SNPs and the stroke in this area has been explored. METHOD:The genotypes of 186 patients with cerebral infarction, 202 patients with primary cerebral hemorrhage and 160 sex- and age-matched heath controls from Han population in Changsha area were detected by PCR-RFLP and direct DNA sequencing. RESULT;The frequencies of the -509C > 7and + 869T > C genotypes and alleles showed no difference between the CI and the control groups, and no difference was found between the CH and the control groups (P > 0. 05) , either. But the frequencies of -509 T and + 869 C carriers in FCI were higher than control group. The stroke prevalences of the -509 T carriers in FCI were 1.557 multiples compared to the control group, while the +869C carriers in FCI were 1.45 multiples compared to the control group. CONCLUSION: TGFBl -509 C > T and + 869T> C are associated with cerebral infarction of people with a family history of cerebral infarction, but have no relationship with cerebral hemorrhage. -509T and +869C alleles are probably the risk factors of stroke of people with history of cerebral infarction.%目的:通过对长沙汉族人群TGFB1的多态分布规律的研究,从遗传流行病学的角度探讨TGFB1SNPs（单核甘酸多态性）与长沙汉族人群脑卒中的关系.方法:应用PCR、RFLP及DNA直接测序等方法对研究人群进行-509C＞T及+869T＞C基因分型.研究对象包括:脑梗死(CI)患者186例,脑出血(CH)患者202例,正常对照人群160例.结果:脑梗死组(CI)和脑出血组(CH)分别与对照组比较,-509C＞T和+ 869T＞C基因型及等位基因频率分布无统计学差异(P＞0.05),有脑梗死家族史的患者（FCI组）与对照组比较,-509 T等位基因携带者及+ 869C等位基因携带者频率较高(P＜0.05),其中-509T携带者脑梗死的患病风险为对照组的1.557倍,+ 869C携带者脑梗死的患病风险为对照组的1.45倍.结论:TGFB1-509C＞T及+869T＞C与有脑梗死家族史的长沙汉族人群脑梗死发病可能相关,但与有脑出血家族史的长沙汉族人群脑出血发病无关,-509T和+ 869C等位基因可能是有脑梗死家族史的长沙汉族人群脑梗死发病的危险因子.
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