目的 以聚乳酸羟基乙酸(PLGA)为载体构建载有骨保护素(OPG)的微球,筛选出缓释效果最佳的制备条件,并研究载药微球的体外释放特性.方法 采用复乳溶剂挥发法,以不同的搅拌速度、聚乙烯醇(PVA)浓度、PLGA浓度制备OPG-PLGA微球并测定其载药量和包封率,通过正交试验优化制备条件;以磷酸盐缓冲液作为释放介质考察载药微球的体外释放特性.结果 以PLGA聚合物浓度400 mg/ml、搅拌速度400 r/min、PVA浓度2％为条件制备的载药微球具有最优的载药量和包封率,分别为6.21×10-和75.10％,体外释药试验显示微球持续释放时间达到30 d,具有良好缓释效果.结论 采用优化条件制备的OPG-PLGA微球具有较高的包封率和载药量,同时具有良好的缓释效果,为用于拔牙位点保存术的缓释药物研究提供了基础.%Objective To screen the optimal preparation conditions of sustained-release drug and reach the release characteristics of drug loaded microspheres,poly lactic acid glycolic acid (PLGA) was used as the carrier to construct the microspheres containing osteoprotegerin (OPG) in this study.Methods The OPG-PLGA microspheres were prepared by double emulsion solvent evaporation method at different stirring speed,polyvinyl alcohol(PVA) concentration and PLGA concentration.Drug loading and entrapment efficiency of the OPG-PLGA microspheres were measured,then the optimum preparation condition was achieved by employing the orthogonal test.The in vitro release characteristics of microspheres were investigated by using PBS as the release medium.Results The drug loaded microspheres with 400 r/min stirring rate,2％ PVA concentration and 400 mg/ml PLGA concentration had the best drug loading and encapsulation efficiency,which were 6.21 × 10-7 and 75.10％,respectively.Besides,in vitro release test showed that the drug loaded microspheres had a good sustained-release effect which sustained release time of 30 days.Conclusion The OPG-PLGA microspheres prepared by the optimized conditions has high drug loading and encapsulation efficiency,and has good sustained-release effect,which provide the basis for the study of the sustained release drugs used in alveolar ridge preservation.
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