目的 探讨维生素B3对PINK1 B9突变转基因果蝇的保护作用及其可能机制.方法 选用TH-Gal4/UAS系统的PD转基因果蝇模型,按处理方式分为正常对照组、PD转基因果蝇模型组、PD转基因果蝇模型+维生素B3干预组,PD转基因果蝇模型用GAPDH2 RNAi基因干预+维生素B3干预组,进行果蝇形态学、ATP水平、果蝇的mRNA水平和蛋白印记检测.结果 适当浓度的维生素B3能部分挽救PINK1B9果蝇的飞行能力和降低翅膀异常率.并且上调了GAPDH和NDUFS3蛋白的表达,增加ATP生成的相对水平.再用GAPDH2 RNAi干预PINK1B9转基因果蝇后GAPDH和NDUFS3蛋白表达相应下降.结论 维生素B3可能通过上调GAPDH和NDUFS3水平发挥调控糖酵解和氧化磷酸化起到保护作用.%Objective To explore the protective effect of vitamin B3 on PINK1B9 transgenic Drosophila and its possible mechanism.Methods The PD-transgenic Drosophila model of TH-Gal4/UAS system was used.They were divided into normal control group,PD transgenic Drosophila melanogaster model group,PD transgenic fruit fly model + vitamin B3 intervention group,PD transgenic fruit fly model and GAPDH2 RNAi gene intervention + vitamin B3 intervention group.The morphology,ATP levels,mRNA levels and protein imprinting of Drosophila melanogaster were examined.Results The appropriate concentration of vitamin B3 could save the capacity of PINK1B9 Drosophila,reduce the rate of abnormal wings,and up-regulate the expression of GAPDH and NDUFS3 proteins.It also increased the relative level of ATP production.The GAPDH and NDUFS3 protein expression were decreased when the GAPDH knockdown(GAPDH2 RNAi) in PINK1B9.Conclusion Vitamin B3 may play a protective role by increasing the levels of GAPDH and NDUFS3 to regulate the glycolysis and oxidative phosphorylation.
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