目的：探讨 PI3K/ AKT 信号通路是否参与芸香苷对过氧化氢(H2 O2)诱导人晶状体上皮细胞(HLEC)凋亡的抑制作用。方法将培养的 HLEC 分为4组：空白对照组、H2 O2组、芸香苷组、LY294002组；采用 MTT 法测细胞存活率,Western blot 法检测 p-AKT 及 AKT 的表达,Annexin V-FITC/ PI 双染流式细胞术检测各组细胞凋亡率。结果H2 O2可诱导 HLEC 发生凋亡,与 H2 O2组相比,芸香苷组不仅使 AKT 的磷酸化水平显著升高,还降低了细胞凋亡率(P<0．01)；在 LY294002干预组, PI3K/ AKT 信号通路的特异性阻断剂 LY294002明显抑制了芸香苷组中各项指标的变化,差异有统计学意义(P <0．01),但与 H2 O2组比较,差异无统计学意义。结论芸香苷抑制 H2 O2诱导的 HLEC 凋亡可能与 PI3K/ AKT 信号通路有关。%Objective To explore whether PI3K/ AKT signaling pathway participates in the inhibiting effect of Ru-tin on H2 O2-induced apoptosis in human lens epithelial cells( HLEC). Methods HLEC were divided into four groups: control group,H2 O2 group,rutin group,LY294002 group. Cell survival rates were determined by a 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay; cell apoptosis rates were monitored by flow cytometry with Annexin V-FITC and propidiun iodide(PI) staining. Western blot was used to measure the expres-sion levels of AKT and p-AKT. Results H2 O2 induced HLEC apoptosis. Compared with H2 O2 group,rutin group not only increased the expression lever of p-AKT,but also reduced cell apoptosis rate(P < 0. 01). In LY294002 group, LY294002, an inhibitor of PI3K/ AKT signaling pathway,could significantly block the change of these inde-xes produced by rutin group(P < 0. 01), but no significant change compared with H2 O2 group. Conclusion Rutin inhibits H2 O2-induced cell apoptosis and may be associated with PI3K/ AKT signaling pathway.
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