Objective To investigate the relationship between the expression of autophagic microtubule-associated protein 1 light chain 3( LC3 ) in pancreas and insulin resistance ( IR ). Methods Twenty rats were randomly divided into control group( C group ), high fat diet group( HF group ) and high fat diet with intervention of chloro-quine group( HFC group ). They were fed for 12 weeks and success to obesity models. The fasting blood glucose ( FBG ) and insulin( Fins ) were monitored and homeostasis model assessment insulin resistance( HOMA-IR ) index were calculated. The expression of autophagic related protein LC3 in pancreas were analyzed by Western blot. Results HOMA-IR index were increased gradually among the groups( P <0. 05 ). The expression of LC3 in HF group was significantly increased as compared with C group, but decreased in HFC group( P <0. 05 ). Conclusion High fat diet may result in insulin resistance and the activation of autophagy in pancreas. The degree of insulin resistance increases significantly after the inhibition of autophagy. Autophagy may be involved in the occurrence of insulin resistance and plays an important role in the pathogenesis of type 2 diabetes mellitus.%目的 探讨大鼠胰腺组织自噬微管相关蛋白1轻链3(LC3)的表达与胰岛素抵抗(IR)的关系.方法 20只大鼠随机分为对照组(C组)、高脂喂养组(HF组)、高脂喂养加氯喹干预组(HFC组),连续喂养12周,造模成功后,动脉采血并留取胰腺组织,测定空腹血糖和胰岛素计算稳态模型胰岛素抵抗(HOMA-IR)指数,Western blot法检测胰腺LC3蛋白表达.结果 3组HOMA-IR逐渐增加(P<0.05);与C组相比,HF组LC3蛋白表达增加,HFC组表达降低(P<0.05).结论 高脂饮食能诱导IR同时激活胰腺组织自噬,而抑制自噬活性后,IR更加明显;自噬可能参与IR,在2型糖尿病的发病中起重要作用.
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