目的：探讨巨噬细胞选择性活化与肥胖相关性胰岛素抵抗间的关系。方法28只3周龄雄性C57BL/6J小鼠，随机平均分为2组：普食组（A组，n＝14只），高脂饮食组（B组，n＝14只），分别给予标准饲料与高脂饲料喂养。6周、12周分别检测空腹血糖（（FBG）、血浆胰岛素（FIns）水平，并计算胰岛素抵抗指数（HOMA-IR），测定肝组织匀浆三酰甘油（TG）、胆固醇（TC）及丙氨酸氨基转移酶（ALT）水平；HE染色观察肝脏及脂肪组织病理学改变，DAB染色及免疫荧光染色检测小鼠肝脏、脂肪组织M2型巨噬细胞的特异性蛋白Ym-1表达情况。结果第6周B组小鼠体质量、FIns、HOMA-IR、TC均明显高于A组，FBG、TG、ALT较A组升高不明显；第12周B组小鼠体质量、FBG、FIns、HOMA-IR、TC、TG和ALT进一步升高，均明显高于A组。第12周B组小鼠脂肪组织内巨噬细胞选择性活化标志Ym-1蛋白表达较A组增强。结论巨噬细胞选择性活化可能参与了肥胖诱导胰岛素抵抗的发生。%Objective To explore the relationship between the selective activation of macrophages and insu -lin resistance.Methods 28 male C57BL/6J mice were randomly assigned into 2 groups.the standard chow group (group A,n=14),mice were fed with basic diet,and high-fat diet-fed group (group B,n=14),mice were t fed with high-fat diet.At 6 and 12 weeks,the levels of fasting peripheral blood glucose (FBG),fasting plasma insulin (FIns) were detected and the levels of triglyceride ( TG) ,cholesterol ( TC) and glutamic pyruvic transaminase ( ALT) were also measured.The changes in adipose tissue and liver were observed .The expression of Ym-1proteinin was detected . Results Compared with group A,the body mass,the level of FIns,HOMA-IR,and TC in group B were significantly increased at the 6th week.However,there were no statistically differences in the levels of FBG ,TG and ALT.The body mass,the levels of FBG,FIns,HOMA-IR,TC,TG and ALT in group B were significantly higher than those in the group A at the 12th week.The expression of macrophages specific marker Ym-1 in adipose tissue was significantly in-creased in group B at the 12th week compared with that in group A .Conclusion Selective activation of macrophage may play a role in the development of obesity-related insulin resistance .
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