目的 研究不同剂量雷帕霉素对小鼠体内Treg细胞的影响.方法 将SPF级昆明系小鼠60只随机分为对照组(A)和实验组(B、C、D),B、C、D三组分别灌胃雷帕霉素1、2、3 mg·kg-1,A组每天予以无菌水灌胃,共3周.3周后,无菌条件下心脏采血,EDTA抗凝,分离脾脏,制备单细胞悬液,采用流式细胞仪检测小鼠外周血和脾脏中CD4+CD25+调节性T细胞水平(CD4+CD25+Treg细胞占CD4+ T细胞的百分比).结果 实验组(B、C、D)小鼠外周血和脾细胞中CD4+CD25+Treg细胞水平分别为(9.62±1.43)%、(13.76±1.97)%、(15.41±2.45)%和(12.23±4.56)%、(23.03±6.18)%、(25.17±6.42)%,对照组(A)小鼠外周血和脾细胞中CD4+CD25+Treg细胞水平分别为(3.52±0.65)%和(6.53±3.01)%,无论是在外周血还是脾细胞中,B、C、D组CD4+CD25+Treg细胞水平明显高于A组(P<0.05),C、D组与B组之间CD4+CD25+Treg细胞水平也有明显差异性 (P<0.05),C组和D组之间CD4+CD25+Treg细胞水平无明显差异性 (P>0.05).结论 雷帕霉素能够诱导昆明系小鼠体内CD4+CD25+Treg细胞增殖,其使用剂量可以影响CD4+CD25+Treg细胞的增殖程度.%Aim To investigate how rapamycin (RAPA) at different doses levels induce Kunming-strain mouse CD4 + CD25 + Treg cells proliferations. Methods 60 Kunming-strain mice at the age of 8 weeks were divided into a control group (A) and three experimental groups (B, C,D). The mice in groups B,C and D were fed RAPA 1 ,2 and 3 mg · kg -1 intragastric administration. The mice in group A were given sterile water as the control group. After three weeks, under sterile conditions by collecting the peripheral blood and then seperating the splenocytes (EDTA anticoagulant) ,we were able to generate a single-cell suspension. The level of CD4 + CD25 + Treg cells in the mouse peripheral blood and splenocytes were detected by flow cytometer. (The ratio of CD4 + CD25 + Treg cells to CD4 + CD25 Treg cells). Results The CD4 + CD25 + Treg cells in the mouse peripheral blood and splenocytes of the experimental groups (B, C, D) were (9.62± 1.43)％ ,(13.76 ± 1.97)％ ,(15.41 ±2.45)％ and (12.23 ±4.56)％ ,(23.03 ±6.18)％ ,(25.17 ± 6.42) ％. The CD4+ CD25 + Treg cells in the mouse peripheral blood and splenocytes of the control group (A) were (3.52 ± 0.65) ％ and (6.53 ± 3.01 ) ％.The levels of the CD4 + CD25 + Treg cells either in the peripheral blood or splenocytes of the groups B, C and D were significantly higher than those in group A (P ＜ 0.05). There were significant differences among group C, D and B according to the level of the CD4 + CD25 + Treg cells (P ＜ 0.05) while there were no significant differences between group C and D according to the level of the CD4 + CD25 + Treg cells (P ＞ 0.05). Conclusion RAPA can induce Kunming-strain mouse CD4 + CD25 + Treg cells proliferations in vivo and the level of CD4 + CD25 + Treg cells proliferations in vivo is influenced by the dose of RAPA used.
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