Aim To develop an HPLC method for simultaneous determination of paeonol, innamic acid and astragaloside in Qizhongyaoshu pills. Methods HPLC-ELSD was used to quantitatively determine the Astragaloside togethor with HPLC-UV for determinating paeonol and c:innamic acid. Results The linear range and recovery showed as follows : for paeonol, the linear range was 0. 123 ～6. 16 μg( r = 0. 999 8 ) , the average recovery was 99. 37％ ; for cmnamic acid ,the linear range was 0. 125 ～ 205μg( r = 0. 999 2 ) , the average recovery was 98. 36％ ; for astragaloside, the linear range was 10. 1～53. 8 μg, the average recovery was 96. 18％ . Conclusion The method is simple , accurate. , and reproducible .and could he effectively used for quality control of Qizhongyaoshu pills.%目的 建立HPLC法同时测定芪仲腰舒丸中丹皮酚、肉桂酸、黄芪甲苷含量的方法.方法 采用HPLC-DAD对该方中的肉桂酸、丹皮酚进行含量测定,流动相为乙腈-0.2%磷酸(25:75).检测波长282 nm,流速流速1.0 ml·min-1;采用HPLC-ELSD对该方中的黄芪甲苷进行含量测定,流动相乙腈-水(30:70),流速0.4 ml·min-1.结果 肉桂酸在0.125～205 μg 范围内成线性关系,r=0.999 2,平均回收率为98.36%(RSD=1.8%,n=6);丹皮酚在0.123～6.16 μg范围内成线性关系,r=0.999 8,平均回收率为99.37%(RSD=1.2%,n=6);黄芪甲苷在10.1～53.8 μg范围内呈对数线性关系,平均回收率为96.18%(RSD= 0.86%,n=6).结论 该方法简便,快速,重复性好,可用于芪仲腰舒丸的质量控制.
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